| 利用近等基因系研究豌豆铁蛋白基因对水稻重要生物学特性的影响 |
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| 引用本文： | 赵霏,任三娟,郭泽建,沈圣泉.利用近等基因系研究豌豆铁蛋白基因对水稻重要生物学特性的影响[J].农业生物技术学报,2011,19(1). |
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| 作者姓名： | 赵霏 任三娟 郭泽建 沈圣泉 |
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| 作者单位： | 1. 浙江大学农业与生物技术学院,杭州,310029
2. 中国农业大学植物病理系,北京,100094 |
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| 基金项目： | 浙江省科技攻关项目(No.2008C22075); 农业部行业专项(No.200803034); 浙江省“8812”计划专项(No.2007C12020-1)资助 |
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| 摘 要： | 为研究外源豌豆铁蛋白基因(Pea-Fer)导入水稻后对受体重要生物学特性的影响,本实验以原始受体亲本粳稻(Oryza sativa ssp.japanica)品种秀水11为轮回亲本,外源豌豆(Pisum sativum)铁蛋白转基因纯系Fer34为非轮回亲本,采取连续回交,结合gus标记基因辅助选择技术,在BC6F3获得含Pea-Fer的Fer34-XS11,它与秀水11构成一对近等基因系。结果显示,(1)Pea-Fer导入显著提高了水稻籽粒中的铁元素含量,但对镁、钙、锰和锌等元素的积累作用影响不大;(2)Pea-Fer导入对种子发芽特性和苗期植株生长速率无显著影响;(3)Pea-Fer导入没有改变成株叶片光合色素的含量和组成,但一定程度上提高了植株的净光合速率;(4)Pea-Fer导入对水稻的成熟期农艺性状及经济性状均无不良影响。外源豌豆铁蛋白基因的导入除使水稻籽粒的铁含量有明显增加外,研究结果表明对水稻其它重要生物学性状并无明显负作用,这为开展富铁水稻新品种培育提供了依据。
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| 关 键 词： | 水稻 豌豆铁蛋白基因(Pea-Fer) 近等基因系 生物学特性 光合生理 |
Effects of Exogenous Pea-ferritin Gene on the Important Biological Features Of Rice Using Near Isogenic Lines |
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| Zhao Fei,Ren Sanjuan,Guo Zejian,Shen Shengquan.Effects of Exogenous Pea-ferritin Gene on the Important Biological Features Of Rice Using Near Isogenic Lines[J].Journal of Agricultural Biotechnology,2011,19(1). |
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| Authors: | Zhao Fei Ren Sanjuan Guo Zejian Shen Shengquan |
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| Institution: | Zhao Fei1 Ren Sanjuan1 Guo Zejian2 Shen Shengquan1 1 College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China,2 Department of Plant Pathology,China Agricultural Academy of Agricultural University,Beijing 100094 |
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| Abstract: | In the present study,we investigated the effects of exogenous pea ferritin gene (Pea-Fer) on the important biological properties of transgenic rice. Fer34-XS11 was obtained from a near isogenic line (NIL BC6F3) of donor parent (exogenous ferritin transgenic pure lines Fer34) and recurrent parent (Oryza sativa ssp.japanica) (Xiushui 11) by successive backcrossing and GUS analysis. Several important biological properties were investigated completely using Fer34-XS11 and Xiushui 11. The major results are as fo... |
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| Keywords: | Rice Pear-Ferritin gene (Pea-Fer) Near isogenic lines Biological properties Photosynthesis |
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