| 家蚕肠道产蛋白酶菌株的分离与鉴定及其发酵条件 |
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| 引用本文： | 王在贵,杨文静,刘朝良,朱保建,魏国清,邹昌瑞.家蚕肠道产蛋白酶菌株的分离与鉴定及其发酵条件[J].农业生物技术学报,2011,19(1). |
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| 作者姓名： | 王在贵 杨文静 刘朝良 朱保建 魏国清 邹昌瑞 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230036 |
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| 基金项目： | 安徽省国际合作项目(No.08080703017); 安徽省人才专项基金(No.2008Z022); 安徽高校省级自然科学研究重点项目(No.KJ2009A031Z); 现代农业产业技术体系建设专项资金资助资助 |
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| 摘 要： | 为探明家蚕肠道中产蛋白酶细菌的种类分布及其作用效果,本研究以家蚕(Bombyx mori)4龄幼虫肠道内容物为材料,采用牛肉膏蛋白胨培养基和酪蛋白培养基分离筛选产蛋白酶细菌菌株,利用16SrDNA序列分析鉴定其种属,并研究其产酶能力和产酶活力较高菌株的最适发酵条件。结果获得3株产蛋白酶菌株皓月NA1、951NA3和951NA6,均归属于不动杆菌属(Acinetobacter),其中皓月NA1号细菌产酶能力最强,最佳产酶量为29.5U/mL。以玉米粉10000mg/L、黄豆粉10000mg/L、MgSO4400mg/L、NaC115000mg/L和K2HPO41000mg/L作为发酵培养基成分,在35℃、起始pH9.0、装液量为80mL/150mL、180r/min振荡培养48h的优化发酵条件下,皓月NA1号细菌最大产酶量可达50U/m L。研究结果对家蚕肠道微生物的分布及肠道益生菌的作用效果调控有着十分重要的意义。
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| 关 键 词： | 家蚕 肠道细菌 蛋白酶 16SrDNA 发酵条件 |
Isolation and Identification of the Protease-producing Bacteria from Gut of Silkworm(Bombyx mori)and Its Suitable Fermentation Condition |
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| Wang Zaigui,Yang Wenjing,Liu Chaoliang,Zhu Baojian,Wei Guoqing,Zou Changrui.Isolation and Identification of the Protease-producing Bacteria from Gut of Silkworm(Bombyx mori)and Its Suitable Fermentation Condition[J].Journal of Agricultural Biotechnology,2011,19(1). |
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| Authors: | Wang Zaigui Yang Wenjing Liu Chaoliang Zhu Baojian Wei Guoqing Zou Changrui |
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| Institution: | Wang Zaigui Yang Wenjing Liu Chaoliang Zhu Baojian Wei Guoqing Zou Changrui Department of Life Sciences,Anhui Agricultural University,Hefei 230036,China |
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| Abstract: | To explore the species distribution and probiotics function of enzyme-producing bacteria in gut of the silkworm,protease-producing bacteria strains were isolated from the inclusion in 4th larval guts of the silkworm Bombyx mori by NA mediums and casein-NA mediums and 16 Sr DNA sequences were used to identify the species. Meanwhile,the enzyme producing activities of the three strains and optimum fermentation conditions of Haoyue NA1 strain were investigated. The results showed that three strains Haoyue NA1,9... |
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| Keywords: | Bombyx mori Gut bacteria Protease 16SrDNA Fermentation conditions |
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