| 影响中苜2号苜蓿愈伤组织诱导的主要因素分析 |
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| 引用本文： | 李芳,赵金梅,石凤翎,王东娟,郝虎东,郭庆海,毕静.影响中苜2号苜蓿愈伤组织诱导的主要因素分析[J].中国草地学报,2009,31(4). |
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| 作者姓名： | 李芳 赵金梅 石凤翎 王东娟 郝虎东 郭庆海 毕静 |
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| 作者单位： | 1. 内蒙古农业大学生态环境学院,内蒙古,呼和浩特,010019
2. 中国农业科学院草原研究所/农业部草原资源与生态重点开放实验室,内蒙古,呼和浩特,010010 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(中国农业科学院草原研究所)项目，奶业专项课题 |
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| 摘 要： | 以MS为基本培养基,采用L16(45)正交试验设计,研究不同外植体类型及植物生长调节物质对中苜2号愈伤组织生长情况、诱导率的影响,筛选适合中苜2号愈伤组织诱导的最佳外植体类型和最优激素配比.结果表明.以下胚轴为外植体诱导的愈伤组织生长状况良好,诱导率高,为诱导愈伤组织的最佳外植体;2,4-D浓度是影响愈伤组织诱导率的最主要因素,其次是外植体类型;诱导中苜2号愈伤组织的最佳培养基为:MS+2,4-D 0.2mg/L+NAA 0.01mg/L+6-BA 1mg/L+KT 0.2mg/L.
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| 关 键 词： | 紫花苜蓿 愈伤组织 诱导 影响因素 |
Analysis of Main Factors Influencing Callus Induction of Alfalfa "Zhongmu No. 2" |
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| LI Fang,ZHAO Jin-mei,SHI Feng-ling,WANG Dong-juan,HAO Hu-dong,GUO Qing-hai,BI Jing.Analysis of Main Factors Influencing Callus Induction of Alfalfa "Zhongmu No. 2"[J].Chinese Journal of Grassland,2009,31(4). |
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| Authors: | LI Fang ZHAO Jin-mei SHI Feng-ling WANG Dong-juan HAO Hu-dong GUO Qing-hai BI Jing |
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| Abstract: | Taking MS as basic medium the effect of different explants types of alfalfa "Zhongmu No. 2" and various plant regulators on callus growth, callus induction and callus browning were investigated with orthogonal test design method in order to choose the best explants and the most optimal plant regulators that were beneficial to callus induction. The results showed that the most helpful explants for callus induc- tion was hypocotyls. 2,4-D was the main factor effecting the callus induction, second is different explants types. The optimal medium for callus induction of alfalfa "Zhongmu No. 2" was MS+2,4-D(0.2rag/L)+ NAA(0.01mg/L) + 6-BA(1mg/L) + KT(0.2mg/L). |
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| Keywords: | Medicago sativa L Callus Induction Influencing factors |
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