| 伪狂犬病病毒Bartha-K61株UL49基因的克隆和序列分析 |
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| 引用本文： | 刘建华,李娜,仇华吉,田志军,童光志.伪狂犬病病毒Bartha-K61株UL49基因的克隆和序列分析[J].中国预防兽医学报,2005,27(4):269-272. |
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| 作者姓名： | 刘建华 李娜 仇华吉 田志军 童光志 |
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| 作者单位： | 1. 中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001;新疆农业大学,动物医学院,新疆,乌鲁木齐,830052
2. 中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 根据已经发表的伪狂犬病病毒(PRV)国内Ea株UL49基因序列,设计并合成了1对引物,通过PCR方法扩增到了PRV Bartha-K61株UL49基因的编码区,并克隆到pMD18-T载体中.重组质粒pMD-UL49经XhoI酶切和PCR鉴定后,进行了序列测定和分析.结果表明,重组质粒pMD-UL49含有PRV Bartha-K61株UL49基因的编码区.同源性分析显示,PRV Bartha-K61株UL49基因序列与国外Kaplan株、国内Ea株相应基因的核苷酸同源性分别为98.9 %和94.1 %,推导的氨基酸序列同源性分别为96.7 %和87.2 %.
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| 关 键 词： | 伪狂犬病病毒 UL49基因 克隆 序列分析 |
| 文章编号： | 1005-0269(2005)02-0269-04 |
| 修稿时间： | 2004年3月29日 |
Cloning and sequencing of the UL49 gene of pseudorabies virus Bartha-K61 strain |
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| LIU Jian-hua,LI Na,QIU Hua-ji,TIAN Zhi-jun,TONG Guang-zhi.Cloning and sequencing of the UL49 gene of pseudorabies virus Bartha-K61 strain[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(4):269-272. |
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| Authors: | LIU Jian-hua LI Na QIU Hua-ji TIAN Zhi-jun TONG Guang-zhi |
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| Abstract: | According to the published sequence of UL49 gene of pseudorabies virus ( PRV) Ea strain,a pair of primers were designed to amplify the UL49 gene of pseudorabies virus (PRV) Bartha_K61 strain.The UL49 gene was amplified by polymerase chain reaction (PCR),subsequently cloned into pMD18_T vector.The recombinant plasmid pMD_UL49 was identified by PCR and Xho I restrictive digestion,then sequenced. The seqencing result showed that the recombinant plasmid pMD_UL49 contained the UL49 gene of 750 bp.The nucleotide homology of UL49 gene between Bartha_K61 and Kaplan and Ea strain was 98.9 % and 94.1 %,respectively.The deduced amino acid homology of UL49 gene was 96.7 % and 87.2%,respectively. |
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| Keywords: | pseudorabies virus UL49 gene cloning sequence analysis |
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