| 花生防御素基因的克隆及原核表达 |
| |
| 引用本文： | 李万福,;刘海燕,;钟旎,;梁炫强.花生防御素基因的克隆及原核表达[J].广西农业生物科学,2009(4):645-650. |
| |
| 作者姓名： | 李万福 ;刘海燕 ;钟旎 ;梁炫强 |
| |
| 作者单位： | [1]广东省农业科学院作物研究所,广州510631; [2]华南师范大学生命科学学院,广州510640 |
| |
| 基金项目： | 本研究由国家高技术研究发展计划（2006AA10Z156）、现代农业产业技术体系建设专项资金和广东省自然科学基金重点项目（07117967）共同资助 |
| |
| 摘 要： | 植物防御素是植物免疫系统的一员，具有防御病原菌侵染植物的功能。本文采用RACE法成功地克隆花生防御素基因，并对其原核表达蛋白进行研究。研究结果表明，采用RACE方法克隆花生防御素基因（AhORRP），得到的cDNA全长为585bp，含一个225bp的开放阅读框（open reading frame，ORF），编码75个氨基酸；经同源分析，花生防御素蛋白与其它物种具有33％～70％的同源性。原核表达获得大小约28kD AhDRRP融合蛋白。该基因的克隆及其原核表达融合蛋白的获取为花生防御素功能鉴定打下一定基础。
|
| 关 键 词： | 花生 植物防御素 克隆 原核表达 |
Cloning and Prokaryotic Expression of Defensin Gene from Peanut （Arachis hypogaea L.） |
| |
| Institution: | Li Wanfu, Liu Haiyan, Zhong ni, Liang Xuanqiang (1. Crops Research Institute, Guangdong Academy of Agriculture Sciences, Guangzhou, 510631; 2. College of Life Science, South China Normal University, Guangzhou, 510640) |
| |
| Abstract: | The plant defensin is one of the plant immune system members, which can defense pathogenic bacteria to invade the plant. To characterize the function of the defensin in peanut, we have isolated defensin gene by RACE and named A hDRRP （gbDQ296045. 1） and have been expressed the A hDRRP in Rostteta in this paper. As a result, the AhDRRP gene was 585 bp in full-length with an 255 bp open reading frame, and it encoded a 75-amino-acid polypeptide; by the homology analysis, peanut defense protein and other species have 33%-70% homology. Obtained the fusion protein AhDRRP was 28 kD by prokaryotic expression. The cloning expression of the fusion protein to obtain for to lay the groundwork cloning and and prokaryotic expressing of the fusion protein AhDRRP will provide foundation for the identification ofpeanut-defensins function. |
| |
| Keywords: | Peanut （A rachis hypogaea L ） Plant defensin Cloning Prokaryotic expression |
| 本文献已被 维普 等数据库收录！ |
|
