Polarization of chemoattractant receptor signaling during neutrophil chemotaxis |
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| Authors: | Servant G Weiner O D Herzmark P Balla T Sedat J W Bourne H R |
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| Institution: | Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94143, USA. |
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| Abstract: | Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile, indicating that it requires activity of one or more Rho guanosine triphosphatases. |
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