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| 谷子盐溶蛋白A-PAGE电泳方法的探索 | |
| 引用本文： | 张华文,杨延兵,秦岭,管延安,段乃彬,倪大鹏,于东安.谷子盐溶蛋白A-PAGE电泳方法的探索[J].山东农业科学,2007(5):34-37. |
| 作者姓名： | 张华文 杨延兵 秦岭 管延安 段乃彬 倪大鹏 于东安 |
| 作者单位： | 1. 山东省农业科学院作物研究所,山东,济南,250100 2. 山东省农业科学院原子能农业应用研究所,山东,济南,250100 3. 安徽大学生命科学院 |
| 基金项目： | 农业结构调整技术专项基金;山东省农业科学院青年基金 |
| 摘 要： | 以10个谷子品种为材料,利用A-PAGE的方法,探索了谷子盐溶蛋白分离的适宜实验条件。通过改变H2O2用量、胶浓度、交联度、电极液pH值、进样量及有无浓缩胶等因素,获得了不同盐溶蛋白酸性电泳图谱,经综合分析,提出了分离谷子盐溶蛋白的适宜A-PAGE电泳体系。具体指标为:1 ml胶液中用1μl 3%H2O2,胶浓度13%,交联度4.0%,电极液pH值3.7,进样量控制在20~30μl之间。在有浓缩胶的条件下,盐溶蛋白分离效果好,电泳图谱清晰,分辨率高。 |
| 关 键 词： | 谷子 盐溶蛋白 A-PAGE |
| 文章编号： | 1001-4942（2007）05-0034-04 |
| 修稿时间： | 2007-03-26 |
Optimization of A-PAGE for Isolating Foxtail Millet (Setaria Italica L. Beauv. ) Salt-soluble Proteins |
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| ZHANG Hua-wen,YANG Yan-bing,QIN Ling,GUAN Yan-an,DUAN Nai-bin,NI Da-peng,YU Dong-an.Optimization of A-PAGE for Isolating Foxtail Millet (Setaria Italica L. Beauv. ) Salt-soluble Proteins[J].Shandong Agricultural Sciences,2007(5):34-37. | |
| Authors: | ZHANG Hua-wen YANG Yan-bing QIN Ling GUAN Yan-an DUAN Nai-bin NI Da-peng YU Dong-an |
| Institution: | 1. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100 ,China; 2. Institute for Application of Atomic Energy, Shandong Academy of Agricultural Sciences, Jinan 250100, China |
| Abstract: | The experiment was conducted to optimize the conditions of A-PAGE for isolating salt soluble proteins of foxtail millet.10 foxtail millet varieties were used for A-PAGE.A number of A-PAGE electropherograms were obtained at different levels of dosage of catalyst,gel concentrations,cross-linkages,injecting volumes,pH of gel buffer,and the use of inspissate gel.The optimal conditions for A-PAGE were raised after deliberate investigation.Detailed criteria were as following: dosage of 3% H2O2 1 μl/ml gel,gel concentration 13%,cross-linkages 4%,pH of gel buffer 3.7,and the injecting volumes between 20 and 30 μl.Furthermore,the use of inspissate gel enhanced the resolution of gel electropherograms. |
| Keywords: | A-PAGE |
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