| 木聚糖酶水解制取低聚木糖的研究 |
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| 引用本文： | 徐勇,陈牧,余世袁,勇强,姚春才.木聚糖酶水解制取低聚木糖的研究[J].林产化学与工业,2002,22(2):57-60. |
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| 作者姓名： | 徐勇 陈牧 余世袁 勇强 姚春才 |
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| 作者单位： | 南京林业大学,化学工程学院,江苏,南京,210037 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 0 70 6 36 ) |
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| 摘 要： | 比较了木聚糖酶和纤维素酶水解木聚糖制备低聚木糖的效果，并在10L酶解罐中研究了搅拌速率和酶解时间等因素对木聚糖酶水解的影响。优化了酶解工艺条件，当木聚糖质量浓度为30g/L，木聚糖酶体积用量为1%，搅拌速率180r/min时，酶解2h低聚糖得率可达35.2%。总糖得率为41.9%。产品酶解液中25.9%固形物是聚合度2-5的低聚木糖。
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| 关 键 词： | 木聚糖 低聚木糖 酶水解 木聚糖酶 生产工艺 |
| 文章编号： | 0253-2417(2002)02-0057-04 |
STUDY ON ENZYMATIC HYDROLYSIS OF XYLAN INTO XYLO-OLIGOSACCHARIDE |
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| XU Yong,?CHEN Mu,?YU Shi-yuan,?YONG Qiang,?YAO Chun-cai.STUDY ON ENZYMATIC HYDROLYSIS OF XYLAN INTO XYLO-OLIGOSACCHARIDE[J].Chemistry & Industry of Forest Products,2002,22(2):57-60. |
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| Authors: | XU Yong ?CHEN Mu ?YU Shi-yuan ?YONG Qiang ?YAO Chun-cai |
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| Abstract: | In the preparation of xylo-oligosaccharide by enzymatic hydrolysis of xylan,effects of xylanase and cellulase were compared,the effect of agitation rate and time dependence were studied in 10-litter tank.Results showed that the optimum hydrolytic conditions were:xylan concentration 30 g/L,xylanase loading 1%(volume ratio)and agitation rate 180 r/min,under which yields of xylo-oligosaccharide and total sugar after 2 hours were 35.2% and 41.9% respectively,and oligomeric xylan of DP 2-5 constituted 25.9% of solid in the substrate product. |
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| Keywords: | xylo-oligosaccharide enzymatic hydrolysis xylanase |
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