| 乌桕SRAP-PCR体系优化与引物筛选 |
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| 引用本文： | 彭婵,张新叶,李振芳,王瑞文,王晓光.乌桕SRAP-PCR体系优化与引物筛选[J].湖北林业科技,2011(1):13-17. |
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| 作者姓名： | 彭婵 张新叶 李振芳 王瑞文 王晓光 |
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| 作者单位： | 湖北省林业科学研究院,武汉,430075 |
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| 摘 要： | 乌桕是我国重要木本油料树种,目前有关乌桕SRAP-PCR相关研究罕有报道.笔者以乌桕优树总基因组DNA为模板,建立了乌桕的SRAP最佳反应体系:20μLPCR反应体积系中,2μL10×PCR buffer、0.2mmol/L dNTPs、0.25mmol/L引物、1.5U Taq聚合酶和40ng模板DNA.并从100个...
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| 关 键 词： | 乌桕 SRAP-PCR 体系优化 引物筛选 |
Optimization SRAP-PCR System and Primer Screening of Sapium sebiferum (Linn.) Roxb. |
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| Peng Chan,Zhang Xinye,Li Zhenfang,Wang Ruiwen,Wang Xiaoguang.Optimization SRAP-PCR System and Primer Screening of Sapium sebiferum (Linn.) Roxb.[J].Hubei Forestry Science and Technology,2011(1):13-17. |
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| Authors: | Peng Chan Zhang Xinye Li Zhenfang Wang Ruiwen Wang Xiaoguang |
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| Institution: | Peng Chan Zhang Xinye Li Zhenfang Wang Ruiwen Wang Xiaoguang(Hubei Academy of Forestry Wuhan 430075) |
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| Abstract: | Sapium sebiferum（Linn.） Roxb.is most important of woody oil tree species in China,but the related SRAP-PCR application on it has been hardly reported.Based on the template DNA of superior tree of Sapium sebiferum（Linn.） Roxb.,the optimum SRAP-PCR reaction system for Sapium sebiferum（Linn.） Roxb.was established as follows： 2μL10×PCR Buffer,0.2mmol/L dNTPs,0.25mmol/L primer,1.5U Taq polymerase and 40ng template DNA,the total reaction volume was 20μl.And 27 primer combinations which produced clear bands and with abundant polymorphism were screened from 100 combinations.The optimized SRAP-PCR reaction system and polymorphic primer combinations provide some technical foundation for the further related genetic analysis of Sapium sebiferum（Linn.） Roxb.Resoures. |
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| Keywords: | SRAP-PCR |
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