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| 核型多角体病毒对棉铃虫酯酶和酚氧化酶活性的影响 | |
| 引用本文： | 王晓容,邓海滨,匡石滋,吴洁芳,邢巧梅,连书亭.核型多角体病毒对棉铃虫酯酶和酚氧化酶活性的影响[J].华中农业大学学报,2003,22(6):553-556. |
| 作者姓名： | 王晓容 邓海滨 匡石滋 吴洁芳 邢巧梅 连书亭 |
| 作者单位： | 1. 广东省农业科学院果树研究所,广州,510640 2. 广东省农业科学院果树研究所,广州,510640;中国科学院华南植物研究所,广州,510650 3. 河南省禹州市生物农药厂,禹州,456570 |
| 基金项目： | 河南省高新技术产业化重点项目[豫高新(2001)5号]，广东省星火计划项目(2003B21602)资助 |
| 摘 要： | 对感染核型多角体病毒(nuclear polyhedrosis virus，NPV)的棉铃虫Helicover pa armigera (Huebner)幼虫血淋巴进行了酯酶和酚氧化酶电泳分析。结果表明：2种酶带在感染12h后其活力发生了明显变化；酯酶酶带2d后发生位移，酚氧化酶酶带5d发生位移，且各自建立新的酶系统。酚氧化酶比活力分析结果表明：感染NPV后，1d达到最高点，2～3d处于最低点，4d后回升。 |
| 关 键 词： | 核型多角体病毒 棉铃虫 酯酶 酚氧化酶 酶活性 影响因素 |
| 修稿时间： | 2003年2月17日 |
The Effect of NPV on Esterase and Phenoloxidase Activities from Helicoverpa armigera (Hübner) Hemolymph |
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| Wang Xiaorong Deng Haibin Kuang Shizi Wu Jiefang Xing Qiaomei Lian Shuting.The Effect of NPV on Esterase and Phenoloxidase Activities from Helicoverpa armigera (Hübner) Hemolymph[J].Journal of Huazhong Agricultural University,2003,22(6):553-556. | |
| Authors: | Wang Xiaorong Deng Haibin Kuang Shizi Wu Jiefang Xing Qiaomei Lian Shuting |
| Abstract: | This paper reported the electrophoresie assay of esterase and phenoloxidase activity in Helicoverpa armigera (Hubner) hemolymph which was infected with nuclear polyhedrosis virus (NPV). The result showed as follows that after being infected for 12 h, the activity of these two emzyme bands changed obviously, and the position of esterase bands shifted at 2 d, the phenoloxidase bands shifted at 5 d, and new enzyme systems presented. The result of phenoloxidase activity assay showed that infected by NPV, the activity reached the maximum at 1 d, reached the minimum between 2 d and 3 d,and rise again at 4 d. |
| Keywords: | Helicoverpa armigera (Hubner) nuclear polyhedrosis virus(NPV) esterase phenol-oxidase |
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