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皮肤特异表达c-Myc转基因小鼠的建立与分析
引用本文:任红艳,张兴举,杨述林,崔文涛,唐中林,李奎.皮肤特异表达c-Myc转基因小鼠的建立与分析[J].农业生物技术学报,2008,16(1):37-40.
作者姓名:任红艳  张兴举  杨述林  崔文涛  唐中林  李奎
作者单位:1. 华中农业大学动物遗传育种与繁殖教育部重点实验室,武汉,430070;中国农业科学院北京畜牧兽医研究所基因与细胞工程研究室,北京,100094
2. 中国农业科学院北京畜牧兽医研究所基因与细胞工程研究室,北京,100094
基金项目:国家高技术研究发展计划(863计划) , 国家科技攻关项目 , 国家自然科学基金
摘    要:以CMV-PAAV载体为基础载体,以皮肤特异人K14(人角蛋白14)基因启动子替换原质粒中的CMV启动子后,在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列,构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术,将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中,然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中,共移植了256枚受精卵,6只受体怀孕,最终获得44只成年小鼠。通过PCR和Southern blot分析证明2只为阳性转基因小鼠,转基因阳性率为4%。Southern杂交结果分析显示外源片断是以多拷贝串联重复的形式整合到鼠染色体中

关 键 词:人角蛋白k14    转基因小鼠    c-Myc基因  皮肤特异表达
文章编号:1006-1304(2008)01-0037-04
收稿时间:2007-05-29
修稿时间:2007-07-17

Establishment and Analysis of Transgenic Mice with c-Myc Gene Specially Expressed in the Skin
REN Hong-yan,ZHANG Xing-ju,YANG Shu-lin,CUI Wen-tao,TANG Zhong-lin,LI Kui.Establishment and Analysis of Transgenic Mice with c-Myc Gene Specially Expressed in the Skin[J].Journal of Agricultural Biotechnology,2008,16(1):37-40.
Authors:REN Hong-yan  ZHANG Xing-ju  YANG Shu-lin  CUI Wen-tao  TANG Zhong-lin  LI Kui
Abstract:Recombinant expression vector which efficiently expressed c-Myc gene was made from pCMV-MCS plasmid backbone by inserting a human kertain 14 promoter to replace the CMV promoter, and the c-Myc gene cDNA sequence and GFP gene coding sequence were inserted into the MCS region. Then the linear vector was injected into two pronucleus of C57BL6 syngenic mice zygotes, and 200 injected eggs were implanted into foster mothers, 6 of which were pregnant and bore 44 offspring. PCR and Southern blotting results revealed that 2 offspring were positive wansgenic mice, and tmnsgenic rate was about 4%. Southern blotting results showed that the foreign gene was integrated in mouse chromosomes with multi- inverted repeat sequences.
Keywords:human kertain 14  transgenic mouse  c-Myc gene  skin specific expression
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