2个芍药品种不同花器官香气成分分析

‘巧玲’和‘杨妃出浴’是杂交选育芳香型芍药新品种的重要亲本材料。为了弄清其不同花器官香气成分、含量及其分布规律，进而为芍药花香相关功能基因的分离、鉴定、克隆、时空表达及芳香型芍药分子育种等后续研究提供一定的理论依据。本文以典型芳香型芍药品种‘巧玲’与‘杨妃出浴’为试材，以淡香型芍药品种‘粉玉奴’为对照，采用顶空固相微萃取和气相-质谱联用技术，检测分析其花瓣、瓣化雄蕊、雌蕊、萼片4个不同花器官的香气成分。结果表明:‘杨妃出浴’含有29种香气成分，主要为香叶醇(27.62%)、苯乙醇(18.00%)、3, 7-二甲基-2-辛烯醇(14.56%)、α-蒎烯(9.79%)，鉴于‘粉玉奴’花瓣样品中未能检测到以上香气成分，因此可初步确定香叶醇、苯乙醇、3, 7-二甲基-2-辛烯醇、α-蒎烯等为‘杨妃出浴’花冠释放香气的关键成分。‘巧玲’含有41种香气成分，主要为芳樟醇(30.06%)、桉油精(23.28%)、苯乙醇(22.09%)、乙酸叶醇酯(11.86%)、石竹烯(5.24%)，其中芳樟醇与桉油精含量特别高，对照分析可初步确定‘巧玲’花香主要来源于芳樟醇、苯乙醇、桉油精与石竹烯的贡献。同时，2个品种不同花器官之间香气物质的释放量均表现出明显差异，其中瓣化雄蕊中均为最高，外层花瓣次之，而雌蕊与萼片中较少，与感官上香气的初步判断相吻合。因此，‘巧玲’和‘杨妃出浴’花香成分在不同花器官部位的空间表达上具有明显差异性。 

Analysis of the aroma composition of different flower organs of two peony varieties

'Qiao Ling ' and 'Yang Fei Chuyu ' are important parent hybridization materials for breeding fragrant peony. The aromatic profile of different flower organs can provide a theoretical foundation to isolate, identify, clone and express genes in a fragrant peony molecular breeding program. Test material included typical fragrant peony varieties 'Qiao Ling ' and 'Yang Fei Chuyu ' , as well as the light-scented peony variety 'Fen Yu Nu ' . Headspace solid-phase microextraction and gas chromatography-mass spectrometry ( GC-MS ) were used to analyze the aromatic profile of petals, petaloid stamens, pistils, and sepals. Results showed that 'Yang Fei Chuyu ' contains 29 aroma components, mainly geraniol(27. 62%), phenylethyl alcohol(18. 00%), 3,7-dimethyl-2-octene alcohol(14. 56%), and α-pinene(9. 79%). Aromatic compounds could not be detected in 'Fen Yu Nu' petals, thus the above listed components define 'Yang Fei Chuyu' fragrance. 'Qiao Ling' contains 41 aromatic compounds, mainly linalool ( 30. 06%) , eucalyptol ( 23. 28%) , benzyl ethanol ( 22. 09%) , leaf alcohol acetate (11. 86%), and caryophyllene(5. 24%). Linalool, benzyl ethanol, eucalyptol and caryophyllene are the main source of 'Qiao Ling' fragrance. The release amount of aroma components between the flower organs of 'Yang Fei Chuyu' and 'Qiao Ling' showed obvious differences, highest in petaloid stamens, followed by the outer petals, pistils and sepals. The aromatic profile of 'Yang Fei Chuyu' and 'Qiao Ling' are distinctly different among these flower organs.