| 多基因表达载体KCTB转化宁夏枸杞的研究 |
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| 引用本文： | 朱永兴,曹鹏,许兴,孟青青,赵子丹.多基因表达载体KCTB转化宁夏枸杞的研究[J].中国农学通报,2010,26(9):74-77. |
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| 作者姓名： | 朱永兴 曹鹏 许兴 孟青青 赵子丹 |
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| 作者单位： | 1. 宁夏农业生物技术重点实验室,银川,750000
2. 宁夏大学生命科学院,银川,750000 |
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| 基金项目： | 973项目;宁夏自然基金 |
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| 摘 要： | 为研究多基因表达载体KCTB转化宁夏枸杞，本研究利用农杆菌介导技术，利用叶盘转化法，将构建有GUS、BAR、KC1、CAF14个功能基因的表达载体KCTB转化宁夏枸杞，并获得了23株抗性植株;GUS组织化学染色显示，在转基因植株的根、茎、叶中都有表达，PCR结果显示，分别检测到了BAR、KC1、CAF1基因，说明多基因表达载体KCTB已经完全整合到了宁夏枸杞的基因组中。
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| 关 键 词： | 耕地占补充平衡 耕地占补充平衡 形势 特征 中国 |
| 收稿时间： | 2009-12-21 |
| 修稿时间： | 2010-01-14 |
The transformation of mutigene expressed vector for Lycium barbarum. L |
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| Zhu Yongxing,Cao Peng,Xu Xing,Meng Qingqing,Zhao Zidan.The transformation of mutigene expressed vector for Lycium barbarum. L[J].Chinese Agricultural Science Bulletin,2010,26(9):74-77. |
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| Authors: | Zhu Yongxing Cao Peng Xu Xing Meng Qingqing Zhao Zidan |
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| Institution: | 1The Key Lab of Agricultural Bio-technology of Ningxia, Yingchuan 750000;
2The Academy for Life Science of Ningxia University, Yingchuan 750000 |
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| Abstract: | For researching transformation of Lycium barbarum L., the multigene expressed vector KCTB that constructed with four functional genes. GUS, BAR, KC1, CAF1 was introduced into Lycium barbarum L. mediated with agrobacterium tumefaciens. Analysis of transgenic plant by histochemical staining showed that the GUS was expressed predominately in leaves, and also lesser degree in roots. The result of PCR suggested that BAR, KC1, CAF1 was detected and also the KCTB was contained in genome of Lycium barbarum L.. |
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| Keywords: | Lycium barbarum L Agrobacterium transformationzz |
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