108杨叶片再生体系的建立

[目的]研究欧美杨108离体叶片再生技术,为遗传转化研究提供科学依据。[方法]研究了外植体的消毒方法和不同激素组合对叶片再生和不定芽生根的诱导效果。[结果]茎段外植体消毒的最佳处理为：75%乙醇溶液消毒10s＋0.1%升汞消毒10min,污染率和褐化率均低,成活率较高;叶片诱导分化培养基以MS＋6-BA0.6mg/L＋NAA0.2mg/L效果最好,诱导率100%,平均不定芽诱导数为26.43个;最佳生根培养基为1/2MS＋IBA0.3mg/L或1/2MS＋IAA0.3mg/L,生根率可达100%,生根数分别为4.83条和5.20条。[结论]以108杨叶片为外植体,建立了108杨的高效再生体系。

Establishment of the Regeneration System of Poplar Leaf Explants

[Objective] The scientific reference for the gene transformation of poplar was provided through the research on the regeneration technique of the poplar(Populus×euramericana cv.'Guariento')leaf culturing.[Method] The effect of the disinfection method and plant growth regulator on the bud growth and the regeneration from leaf culture was experimented.[Results] The best disinfection method was the leaf was treated with 75% alcohol solution for 10 seconds,and then,soaked with 0.1% HgCl2 for 10 minutes,with low contamination and browning rate and high survival rate.The best medium for the inducement of bud was MS+6-BA 0.6 mg/L+NAA 0.2 mg/L with the inducement rate:100% and the average rate of bud-growing:26.43 buds.The best rooting medium was 1/2MS+IBA 0.3 mg/L or 1/2MS+IAA 0.3 mg/L,with the root-growing rate:100% and the average number of root:4.83 and 5.20 roots.[Conclusion] The regeneration system of poplar leaf culturing was established.