| 甜叶菊多倍体离体诱导技术体系的建立 |
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| 引用本文： | 王波,崔广荣,何克勤,胡能兵,林平,张子学. 甜叶菊多倍体离体诱导技术体系的建立[J]. 热带作物学报, 2011, 32(9): 1711-1714. DOI: 10.3969/j.issn.1000-2561.2011.09.026 |
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| 作者姓名： | 王波 崔广荣 何克勤 胡能兵 林平 张子学 |
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| 作者单位： | 安徽科技学院植物科学学院,安徽凤阳,233100 |
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| 基金项目： | 安徽省教育厅省级重点自然科学基金项目(No.KJ2010A073); 安徽省甜叶菊品种选育及栽培工程技术研究中心建设项目 |
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| 摘 要： | 以甜叶菊茎段为外植体,用不同浓度的秋水仙素在试管苗茎段再生植株过程中进行不同时间处理,探讨适合于甜叶菊离体多倍体诱导的最佳秋水仙素处理时间和浓度,初步建立甜叶菊离体诱导多倍体的技术体系。结果表明,秋水仙素对甜叶菊植株形成影响较大,秋水仙素处理的最佳剂量为0.1%处理3 d,再生植株总数高且多倍体比率达42.3%;同时,再生苗生根也受到了一定影响;多倍体苗叶片厚实、颜色较深、植株矮化,气孔增大,单位面积气孔数较少;多倍体细胞核体积增大,染色体数加倍。
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| 关 键 词： | 甜叶菊 离体培养 秋水仙素 多倍体 |
Establishment of the Technique System of in vitro Polyploidy Induction for Stevia rebaudiana Bertoni |
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| WANG Bo,CUI Guangrong,HE Keqin,HU Nengbing,LIN Ping and ZHANG Zixue. Establishment of the Technique System of in vitro Polyploidy Induction for Stevia rebaudiana Bertoni[J]. Chinese Journal of Tropical Crops, 2011, 32(9): 1711-1714. DOI: 10.3969/j.issn.1000-2561.2011.09.026 |
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| Authors: | WANG Bo CUI Guangrong HE Keqin HU Nengbing LIN Ping ZHANG Zixue |
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| Affiliation: | WANG Bo,CUI Guangrong,HE Keqin,HU Nengbing,LIN Ping,ZHANG Zixue Plant Science School of Anhui Science and Technology University,Fengyang,Anhui 233100,China |
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| Abstract: | The stem sections of Stevia rebaudiana Bertoni were used as explants for polyploidy induction in vitro with different concentrations of colchicines for different time treatments during shoot formation.It was investigated that the best concentrations of colchicines and suitable treatment time in order to establish the technique system of polyploidy induction in vitro for Stevia rebaudiana Bertoni.There was great effect of colchicines on plantlet formation from stem section.The best treatment of colchicines w... |
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| Keywords: | Stevia rebaudiana Bertoni In vitro culture Colchicines Polyploidy |
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