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| 辣椒小G蛋白CaRab8基因全长cDNA的分离及表达特征的初步分析 | |
| 引用本文： | 赖燕,肖翔,林菁,虞露,陈桂信,官德义,何水林.辣椒小G蛋白CaRab8基因全长cDNA的分离及表达特征的初步分析[J].热带作物学报,2011,32(6):1111-1115. |
| 作者姓名： | 赖燕 肖翔 林菁 虞露 陈桂信 官德义 何水林 |
| 作者单位： | 1. 福建农林大学生命科学学院,福建福州,350002 2. 福建农林大学园艺学院,福建福州,350002 3. 福建农林大学作物学院,福建福州,350002 |
| 基金项目： | 国家自然基金(No.30600391、30971718); 高校博士点基金(No.20093515110004); 福建省自然基金重点项目(No.2008J0003); 省自然基金(No.2008J0049); 省创新人才项目(No.2007F3013); 省科技重点项目(No.2008N0099) |
| 摘 要： | 通过对辣椒均一化cDNA文库的筛选,分离获得了一个与烟草RAB8-2基因编码的蛋白有着高度同源性的cDNA阳性克隆,命名为CaR ab8.测序结果表明,该cDNA长度为961 bp,包含有651 bp的完整的开放阅读框,推测编码一个217个氨基酸的蛋白,该蛋白具有GTP/GDP结合活性必需的保守域和包括YYRGA在内的... |
| 关 键 词： | 辣椒 小G蛋白 实时定量RT-PCR |
Isolation and Primary Expression Analysis of a Full-Length cDNA Clone Encoding Small GTP-Binding Protein Gene CaRab8 in Pepper |
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| LAI Yan,XIAO Xiang,LIN Jing,YU Lu,CHEN Guixin,GUAN Deyi and HE Shuilin.Isolation and Primary Expression Analysis of a Full-Length cDNA Clone Encoding Small GTP-Binding Protein Gene CaRab8 in Pepper[J].Chinese Journal of Tropical Crops,2011,32(6):1111-1115. | |
| Authors: | LAI Yan XIAO Xiang LIN Jing YU Lu CHEN Guixin GUAN Deyi and HE Shuilin |
| Institution: | LAI Yan1,XIAO Xiang1,LIN Jing1,YU Lu1,CHEN Guixin2,GUAN Deyi3,HE Shuilin1 Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002 |
| Abstract: | One 961bp full-length cDNA clone was isolated from pepper normalized cDNA library,which containing a complete 651 bp open reading frame and encoding a putative protein composed of 217 amino acids.Amino acid sequence deduced by this cDNA clone showed high similarity to NtRAB8-2 protein from tobacco.The full-length cDNA was named CaRab8.The protein encoded by this cDNA clone included conserved domains for guanine nucleotide binding and GTPase activities and five domains specific to Rab family including YYRGA ... |
| Keywords: | Pepper Small G protein Real-time quantitative PCR |
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