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| 枇杷试管苗限制生长保存过程中叶肉细胞超微结构变化的研究 | |
| 引用本文： | 周红玲,郑加协,赖钟雄,陈石.枇杷试管苗限制生长保存过程中叶肉细胞超微结构变化的研究[J].热带作物学报,2011,32(8):1479-1482. |
| 作者姓名： | 周红玲 郑加协 赖钟雄 陈石 |
| 作者单位： | 1. 福建省农业科学院闽台园艺研究中心,福建漳州 363005;福建农林大学园艺学院亚热带果树研究所,福建福州 350002 2. 福建省农业科学院闽台园艺研究中心,福建漳州,363005 3. 福建农林大学园艺学院亚热带果树研究所,福建福州,350002 |
| 基金项目： | 国家科技支撑计划(No.2007BAD07B01); 福建省重大科技专项(No.2004NZ 02-2) |
| 摘 要： | 本试验以早钟6号枇杷试管苗为材料,将其分别保存在MS+5 mg/L PP333培养基上和MS培养基(对照)上,采用超薄切片透射电镜对保存的试管苗成熟叶片叶肉细胞进行观察。结果表明:与对照相比,添加5 mg/L的PP333,保存6个月时,明显推迟了叶绿体的变形、移动、叶绿体和线粒体的镶嵌及叶绿体的解体;保存9个月时,叶肉细胞叶绿体结构完整,少数出现解体,淀粉粒明显增大、增多,线粒体和细胞核结构正常。 |
| 关 键 词： | 枇杷 限制生长保存 叶肉细胞 叶绿体 线粒体 超微结构 |
Ultrastructural Changes of Mesophyll Cells during Restricting Growth Preservation in vitro Plantlets of Loquat(Eriobotrya japonica) |
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| ZHOU Hongling,ZHENG Jiaxie,LAI Zhongxiong and CHEN Shi.Ultrastructural Changes of Mesophyll Cells during Restricting Growth Preservation in vitro Plantlets of Loquat(Eriobotrya japonica)[J].Chinese Journal of Tropical Crops,2011,32(8):1479-1482. | |
| Authors: | ZHOU Hongling ZHENG Jiaxie LAI Zhongxiong and CHEN Shi |
| Institution: | ZHOU Hongling1,2,ZHENG Jiaxie1,LAI Zhongxiong2,CHEN Shi1 1 Fujian-Taiwan Horticulture Research Center,Fujian Academy of Agricultural Sciences 2 Institute of Horticulture Biotechnology/Subtropical Fruits,Fujian Agriculture and Forestry University,Fujian,Fuzhou 350002,China |
| Abstract: | The ultrastructural changes of the mesophyll cells in response to in vitro plantlets of the loquat cultivar Zaozhong No.6 preserved on MS+5 mg/L PP333 were investigated.The results showed that the deformation,shift and disintegration of chlorolplasts and the mosaic of mitochondria were significantly delayed during restricting growth preserved for 6 months.The structure of the chloroplasts in the mesophyll cells was intact,only a few of that disintegrated.The starch grain significantly became larger and nume... |
| Keywords: | Loquat(Eriobotrya japonica) Restricting growth preservation Mesophyll cell Chloroplast Mitochondrium Ultrastructure |
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