一种适用于多糖多酚植物的高质量RNA快速提取方法

植物总RNA的提取是进行下游分子生物学实验的关键基础,高质量的RNA是下游实验成功的保障。许多植物由于其体内含有酚、多糖以及其他的一些次生代谢物,要获得这些植物高质量的总RNA比较困难。本文在多次实验的基础上,提出了改良LiCl沉淀法,该方法步骤少、效率高,使用此方法得到的棉花幼苗总RNA经检测表明其质量比使用冷酚法提取的RNA要高得多,且完全满足后续实验的要求。使用此方法成功提取了香蕉叶片、根、木薯叶片的RNA,经检测表明,所得到的RNA完整性好,完全可以满足后续实验的要求,说明改良LiCl沉淀法是一种适用于多酚多糖植物的快速RNA提取方法。

Rapid Isolation of High-quality RNA from High Phenol-, Polysaccharide Plants

High-quality biologically active RNA isolation is crucialin plant molecular biological experiment.Many plants have high levels of endogenous phenolics,polysaccharides,and secondary metabolityes,so isolating high-quality biologically active RNA from these plants is very difficult.In this paper,a modified LiCl RNA preparation method was developed to isolate RNA from cotton young plant,to yields high-quality total RNA.Then the high-quality RNAs were successfully isolated under this method from banana leaf,cass...