ELISA法筛选抗新孢子虫中药的研究

目的]筛选抗新孢子虫效果较好的中药。方法]取健康昆明种小鼠,投服甲基氢化泼尼松,使其免疫力下降,然后随机分组,每只小鼠腹腔接种1.0×104个新孢子虫虫体,接种4 h后,各组小鼠灌服不同中药。给药7 d后,小白鼠眼球采血,收集血清,应用ELISA法检测其抗体水平。结果]15个中药组中有4个中药组抗体阳性率较低。结论]黄芩、百部、天麻、黄连可以提高感染新孢子虫小白鼠的免疫水平。

Screening of Chinese Medicine Resisting Neospora caninum

Objective] The study aimed to screen Chinese medicines with good resistances to Neospora caninum.Method] Healthy Kunming mice pre-treated with methylprednisolone for the purpose of immunity decrease were randomly divided into 15 groups,and each mouse was intraperitoneally inoculated with 1.0×104 N.caninum.Four hours later,the mice were gavaged with various Chinese medicines. Seven days post-administration,eyeball blood sampling was conducted and the serum was used for antibody level detection with ELISA method.Result] There ware 4 among the 15 Chinese medicine groups showed low antibody positive rates.Conclusion] Scutellaria baicalensis,Stemona sessilifolia,Gastrodia elata and Coptis chinensis could enhance the immunity level of mice infected by N.caninum.