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| CD58对猪PBMC转化的作用 | |
| 引用本文： | 靳亚平,王爱华,肖俊杰. CD58对猪PBMC转化的作用[J]. 西北农林科技大学学报(自然科学版), 1998, 26(2): 62-65 |
| 作者姓名： | 靳亚平 王爱华 肖俊杰 |
| 作者单位： | 西北农业大学动物医学系，陕西省畜牧兽医研究所 |
| 摘 要： | 应用ＭＴＴ比色法，测定了由ＳＲＢＣ膜及ＰＲＢＣ膜提取的ＣＤ５８对猪ＰＢＭＣ转化的影响。结果证明，两种ＣＤ５８成分均能显著提高ＰＨＡ－Ｐ对ＰＢＭＣ的激活作用（Ｐ＜０．０１或Ｐ＜０．０５），单独应用也表现出对ＰＢＭＣ的活化效应，且上述作用具有剂量依赖性。ＣＤ５８浓度达２．５ｇ·Ｌ－１时作用最强（Ｐ＜０．０５），超过该浓度则活化作用减弱，其作用的规律与ＲＢＣ一致。比较分析表明，猪源ＣＤ５８在最佳剂量时，单独刺激作用或对ＰＨＡ－Ｐ诱导的ＰＢＭＣ转化的协同作用均显著大于绵羊源ＣＤ５８（Ｐ＜０．０５），ＰＲＢＣ的协同刺激作用也大于ＳＲＢＣ． |
| 关 键 词： | 猪 红细胞 CD58 淋巴细胞 转化 |
Activation of Pig PBMC Induced by CD58 |
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| Jin Yaping Wang Aihua Xiao Junjie Wang Wenke. Activation of Pig PBMC Induced by CD58[J]. Journal of Northwest A&F University（Natural Science Edition）, 1998, 26(2): 62-65 | |
| Authors: | Jin Yaping Wang Aihua Xiao Junjie Wang Wenke |
| Affiliation: | 2 (1 Department of Veterinary Science,Northwestern Agricultural University,Yangling,Shaanxi 712100) (2 Shaanxi Institute of Veterinary and Animal Sciences,Xianyang,Shaanxi 712039) |
| Abstract: | The effects of CD58 from SRBC (sCD58) and pig RBC (PRBC) on proliferation response of pig PBMC are determined by using MTT colorimetric assay.The results show that the CD58 from different animals and in different forms has the most significant or significant coordinating effects on the PHA P inducing proliferation response of pig PBMC ( P <0.01 or P <0.05) and can also induce the PBMC proliferation response independently.The effect is similar to SRBC and PRBC,and has dose dependence as well,the best density of CD58 being 2.5 mg/mL.The results also indicate that the coordinating effect of pig CD58 and PRBC on the PHA P inducing pig PBMC proliferation response is higher than that of sheep CD58 and SRBC ( P <0.05 or P <0.01). |
| Keywords: | pig erythrocyte CD58 PBMC proliferation |
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