| 桃褐腐病菌拮抗细菌的分离筛选与鉴定 |
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| 引用本文： | 胡海文,杨海清,王 朋,刘素花,赵晓燕,刘正坪.桃褐腐病菌拮抗细菌的分离筛选与鉴定[J].中国农学通报,2009,25(12):195-200. |
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| 作者姓名： | 胡海文 杨海清 王 朋 刘素花 赵晓燕 刘正坪 |
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| 作者单位： | 北京农学院植物科学技术系,北京,102206 |
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| 基金项目： | 北京市教委资助项目，北京市教委资助项目，北京市农委资助项目，北京农学院引进人才科研启动基金，北京市科委区县专项基金 |
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| 摘 要： | 为了筛选和鉴定桃园土壤中对桃褐腐病菌有拮抗作用的细菌，本研究采用了对峙培养法，16SrDNA基因分析以及生理生化反应等方法。从北京平谷区桃园土样中分离的42株细菌中筛选出了11株对桃褐腐病菌（Monilinia fructicola）有明显抑制作用的拮抗细菌。其中F3和Y4的拮抗效果最好，抑菌带的宽度分别为13.83 mm和14.67 mm， F1 和F4 抑菌效果次之，抑菌带宽度分别为11.83mm和12.00mm。在显微镜下观察发现，F3和Y4对桃褐腐病菌的菌丝有致畸作用，并且F3和Y4 的培养滤液还能够抑制桃褐腐病菌分生孢子的产生和萌发。经鉴定表明：F1 、F3、F4和Y4均为枯草芽孢杆菌（Bacillus subtilis）
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| 关 键 词： | 拮抗细菌 桃褐腐病菌 分离筛选 16SrDNA 生理生化反应 |
| 收稿时间： | 2009-01-13 |
| 修稿时间： | 2009-03-26 |
Isolation and Screening of Antagonistic Bacteria against Monilinia fructicola |
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| Hu Haiwen,Yang Haiqing,Wang Peng,Liu Suhu,Zhao Xiaoyan,Liu Zhengping.Isolation and Screening of Antagonistic Bacteria against Monilinia fructicola[J].Chinese Agricultural Science Bulletin,2009,25(12):195-200. |
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| Authors: | Hu Haiwen Yang Haiqing Wang Peng Liu Suhu Zhao Xiaoyan Liu Zhengping |
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| Institution: | (Dapartment of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206) |
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| Abstract: | Confrontation incubation, 16s rDNA analyse and physiological and biochemical reactions were adopted to screen and identificate the antagonistic bacteria against Monilinia fructicola from peach orchard soil. 11out of 42 bacteria strains isolated from peach orchard soil samples of Pinggu strict, Beijing showed obvious antagonism against Monilinia fructicola. F3 and Y4 had the best antagonistic effects with the antagonistis strip width of 13.83mm and 14.67mm separately. The antagonistic effects of F1 and F4 came second with the anatagonistis strip width of 11.83mm and 12.00mm. F3 and Y4 had teratogenicity to the mycelium morphology of Monilinia fructicola. And the culture filtrate of F3 and Y4 could inhibit the conidial formation and germination of M. fructicola. F1 、F3、F4 and Y4 were identified as Bacillus subtilis. |
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| Keywords: | 16SrDNA |
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