淮南猪遗传特异性的RAPD分析

试验用150个10碱基随机引物对引入品种杜洛克猪、长白猪、大约克猪及河南地方品种淮南猪4个猪种基因组池DNA进行了RAPD分析。电泳检测结果发现,其中72个引物扩增出明显的多态性条带,共检测到911条扩增片段,其中多态性片段462条,占50.71%。统计分析结果表明,大约克猪与长白猪之间的遗传距离指数为0.0064,遗传关系最近;淮南猪与杜洛克猪、大约克猪遗传距离指数相近,分别为0.0068、0.0069,遗传关系较近;淮南猪与长白猪遗传距离指数为0.0072,遗传关系最远。结果显示,河南省地方品种淮南猪与其他引入品种之间有遗传的相似性,也存在差异,同时也证明了RAPD技术可以作为分子标记,很好地检测猪种群内的遗传变异及区分不同猪种的遗传检测方法。

RAPD Analysis of the Genetic Specificity of Huainan Pig

Genetic relationship among Duroc, Landrace and Large white were analyzed with random amplified polymorphic DNA (RAPD) technique. 150 random primers were used to amplify genomic DNA of pig with PCR method. On the basis of preliminary experiments, 72 polymorphic primers were selected to analyze the genetic variation among 4 pig lines, 911 amplified fragments were detected by agarose gel electrophoresis. Among them,462 fragments were polymorphic, accounting for 50.71% of the whole. The results showed that the RAPD polymorphisms among pig strains were abundant. The results of the statistical analysis of RAPD bands showed that the genetic similarity were 0.0068 and 0.0069 in Huainan pig and Duroc, Landrace，which indicated that Huainan pig had a closer relationship with Duroc, Landrace, respectively, and the genetic similarity between Huainan pig and Large white was 0.0072, which showed the Huainan pig were cleary apart from Large white. The amplified band patterns showed that there were both genetic similarities and genetic variations among Duroc, Landrace, Large white and Huainan pig. Thus, the RAPD technique could be used to monitor genetic relationship among different strains and different individuals of the same strain of pig.