|
|||||
|
|
| 香蕉Maasr1基因RNAi植物表达载体的构建及鉴定 | |
| 引用本文： | 董凤英,杨超,陈焕雄,张建斌,徐碧玉,金志强.香蕉Maasr1基因RNAi植物表达载体的构建及鉴定[J].热带作物学报,2009,30(3):332-337. |
| 作者姓名： | 董凤英 杨超 陈焕雄 张建斌 徐碧玉 金志强 |
| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;海南大学农学院,海口,570228 2. 琼海市加积中学,海南琼海,571400 3. 海南绿晨香蕉研究所,海口,570216 4. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 |
| 基金项目： | 公益性行业(农业)科研专项基金，中央级公益性科研院所基本科研业务费专项资金资助项目 |
| 摘 要： | 以香蕉Maasr1基因为目的基因,载体pBS为中间载体,pBBB、pBI121为表达载体,分别构建含有Maasr1基因的RNAi植物表达载体DBBB-S-I-A和含有Maasr1基因正义片段的植物表达载体pBl121-S,并通过农杆菌介导的方式对构建的该RNAi载体沉默效果进行鉴定.结果表明:(1)利用中间载体pBS和植物表达载体pBBB成功构建了香蕉Maasr1基因的RNAi植物表达载体pBBB-S-I-A;(2)利用载体pBI121成功构建了1个含有Maasr1基因正义片段的植物表达载体pBI121-S:(3)构建的RNAi载体pBBB-S-I-A可以抑制Maasr1基因的表达,具有较好的沉默效果. |
| 关 键 词： | 香蕉 Momr1基因 RNAi植物表达载体 鉴定 |
Construction and Identification of a RNAi Vector of Maasr1 gene |
|
| Dong Fengying,Yang Chao,Chen Huanxiong,Zhang Jianbin,Xu Biyu and Jin Zhiqiang.Construction and Identification of a RNAi Vector of Maasr1 gene[J].Chinese Journal of Tropical Crops,2009,30(3):332-337. | |
| Authors: | Dong Fengying Yang Chao Chen Huanxiong Zhang Jianbin Xu Biyu and Jin Zhiqiang |
| Institution: | Dong Fengying1,2,Yang Chao3,Chen Huanxiong4,Zhang Jianbin1,Xu Biyu1,Jin Zhiqiang1 1 Institute of Tropical Bioscience , Biotechnology / Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571101,2 College of Agronomy,Hainan University,Haikou 570228,3 Jiaji Middle School,Qionghai 571400,4 Hainan Luchen Banana Institute,Haikou 570216 |
| Abstract: | A RNAi vector named as pBBB-S-I-A was constructed from plasmids pBS and pBBB, and a plant expression vector named as pBI121-S from Maasr1 gene and Gus gene. The vector pBI121-S was then co-transformed with the RNAi vector pBBB-S-I-A. GUS histochemical assay showed that the RNAi vector interfered in the expression of Maasr1 gene. The result demonstrates that the RNAi vector is effective for gene silencing. |
| Keywords: | banana(Musa acuminate) Maasr1 gene RNAi vector identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《热带作物学报》浏览原始摘要信息 | |
| 点击此处可从《热带作物学报》下载免费的PDF全文 | |