Fruit set,seed development and embryo germination in interploid crosses of citrus |
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| Affiliation: | 1. Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, Pakistan;2. College of Agriculture, Sultan Qaboos University, P.O. Box 34, PC-123, Muscat, Sultanate of Oman;1. Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, Punjab, Pakistan;2. Department of Crop Physiology, University of Agriculture, Faisalabad, Pakistan;3. Plant Breeding Institute, University of Sydney, 107 Cobbitty Rd., Cobbitty 2570, NSW, Australia;1. Consiglio per la Ricerca in agricoltura e l’analisi dell’Economia Agraria – centro di ricerca per l’Agrumicoltura e le Colture Mediterranee (CREA-ACM), Corso Savoia 190, 95024 Acireale, Italy;2. Dipartimento D3A, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy;1. Departamento de Zoologia, Universidade Estadual Paulista, Av. 24 A, 1515, CP199, Rio Claro, SP, 13506-900, Brazil;2. Departamento de Botânica, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG, 31270-901, Brazil;3. Division of Biological Sciences, The University of Montana, 32 Campus Dr, Missoula, MT, 59812, USA;1. Laboratoire Biochimie et Biologie Moléculaire du Végétal, CNRS, UMR 6134 SPE, Université de Corse, Corte, France;2. Laboratoire Parasites et Ecosystèmes Méditerranéens", CNRS, UMR 6134 SPE, Université de Corse, Corte, France;3. Equipe "Amélioration des Plantes à Multiplication Végétative", UMR AGAP, Département BIOS, CIRAD, Station de Roujol, Petit-Bourg, Guadeloupe;4. UCA, INRA, PIAF, Clermont-Ferrand, France;5. UMR AGAP Corse, station INRA/CIRAD, San Giuliano, France;1. College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, PR China;2. College of Plant protection, Shenyang Agricultural University, Shenyang, Liaoning 110866, PR China;1. Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan;2. Centre of Agricultural Biochemistry & Biotechnology, University of Agriculture, Faisalabad, Pakistan |
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| Abstract: | Interploid crosses of diploid Kinnow mandarin, Succari sweet orange and Sweet lime with tetraploid Kinnow were made reciprocally for the production of triploid plants. Low fruit set and occurrence of underdeveloped or empty seeds was common in all interploid crosses. The hybrid fruits harvested after 12–14 weeks or 7 months after pollination produced many underdeveloped and few developed seeds; however, tetraploid Kinnow as seed parent yielded more developed seeds per fruit. The under developed seeds were devoid of endosperm. Nucellar embryony was higher in the diploid than in the tetraploid strain of Kinnow. Immature embryos of seeds harvested 12–14 weeks after pollination were cultured in vitro on MS media supplemented with adenine sulphate (20 mg l−1) and malt extract (500 mg l−1). Maximum germination (75%) of hybrid embryos from underdeveloped seeds was obtained in 4× × 2× cross of Kinnow strains. The germinated plantlets were transferred into pots and noted 59–89.3% survival rate in various crosses. |
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