Fibronectin modified expression of Sonic hedgehog in ATRA-mediated neuronal
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| Authors: | Kazuhiko ARAHARA Takashi MATSUMOTO Fumiki MORIMATSU Katsuhiko ARAI |
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| Affiliation: | 1)Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan;2)R&D Center, Nippon Meat Packers, Inc., Tsukuba, Ibaraki, 300-2646, Japan |
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| Abstract: | In this study, the effect of fibronectin on the neurite outgrowth from embryoidbodies (EBs) in neurodifferentiated embryonal carcinoma P19 cells was examined. Theneurite outgrowth on fibronectin was maintained for a longer time in comparison with thoseon collagen or laminin. Quantitative RT-PCR revealed that mRNA level corresponding tosonic hedgehog (Shh) in neurodifferentiated P19 cells was upregulated on fibronectin,whereas collagen or laminin did not affect. Further knockdown of integrin αv subunit inP19 cells demonstrated that expression of Shh was mediated through interaction betweenfibronectin and integrin. Additionally, exogenous Shh agonist accelerated neuriteoutgrowth from embryonic stem cell-derived EBs without large change of neuronal phenotypeexpression. Taken together, fibronectin could maintain neurite outgrowth via increased Shhexpression. |
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| Keywords: | fibronectin integrin neuronal differentiation retinoic acid Shh |
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