Fibronectin modified expression of Sonic hedgehog in ATRA-mediated neuronal
differentiation |
| |
Authors: | Kazuhiko ARAHARA Takashi MATSUMOTO Fumiki MORIMATSU Katsuhiko ARAI |
| |
Institution: | 1)Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan;2)R&D Center, Nippon Meat Packers, Inc., Tsukuba, Ibaraki, 300-2646, Japan |
| |
Abstract: | In this study, the effect of fibronectin on the neurite outgrowth from embryoid
bodies (EBs) in neurodifferentiated embryonal carcinoma P19 cells was examined. The
neurite outgrowth on fibronectin was maintained for a longer time in comparison with those
on collagen or laminin. Quantitative RT-PCR revealed that mRNA level corresponding to
sonic hedgehog (Shh) in neurodifferentiated P19 cells was upregulated on fibronectin,
whereas collagen or laminin did not affect. Further knockdown of integrin αv subunit in
P19 cells demonstrated that expression of Shh was mediated through interaction between
fibronectin and integrin. Additionally, exogenous Shh agonist accelerated neurite
outgrowth from embryonic stem cell-derived EBs without large change of neuronal phenotype
expression. Taken together, fibronectin could maintain neurite outgrowth via increased Shh
expression. |
| |
Keywords: | fibronectin integrin neuronal differentiation retinoic acid Shh |
|
|