LC-MS/MS measurement of ampicillin residue in swine tissues at 5 days after
in-feed administration |
| |
Authors: | Kouko HAMAMOTO Yasuharu MIZUNO |
| |
Institution: | 1)National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1–15–1 Tokura, Kokubunji, Tokyo 185–8511, Japan |
| |
Abstract: | We assessed ampicillin (ABPC) concentrations of kidney, muscle and intestine
after a 5–day withdrawal period in 2 male and a female young Large White pigs fed the diet
containing ABPC (ABPC medicated feed, 24 mg/kg/day) for a week. The ABPC residues were
measured with liquid chromatography–tandem mass spectrometry, and the mean recoveries and
quantitation limits ranged from 91.8 to 97.2% and from 0.1 to 0.12 ng/g,
respectively. The residual ABPC concentrations were ≤1.18 ng/g for the
muscle, ≤0.53 ng/g for the kidney and ≤1.93 ng/g for the
intestine, suggesting below the Japanese provisional maximum residue limits. These results
reveal that the analytical method is developed for residual ABPC and that the withdrawal
period is appropriate. |
| |
Keywords: | ampicillin LC– MS/MS medicated feed residue level swine tissue |
|
|