Molecular epidemiological characterization of poultry red mite,
Dermanyssus gallinae,in Japan |
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Authors: | Thi Thanh Huong CHU Takako MURANO Yukiko UNO Tatsufumi USUI Tsuyoshi YAMAGUCHI |
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Institution: | 1) Laboratory of Veterinary Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4–101 Koyama Minami, Tottori 680–8553, Japan;2) Chiba Prefectural Livestock Research Center, He 16–1 Yachimata, Yachimata, Chiba 289–1113, Japan;3) Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, 4–101 Koyama Minami, Tottori 680–8553, Japan |
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Abstract: | Dermanyssus gallinae, the poultry red mite, is an obligatory
blood-sucking ectoparasite. The genetic diversity of D. gallinae has been
examined in some countries, but so far not in Asian countries. Here, we sequenced a part
of the mitochondrial cytochrome oxidase subunit I (COI) and16S rRNA genes and nuclear
internal transcribed spacers (ITS) region in 239 mite samples collected from 40
prefectures throughout Japan. The COI and 16S rRNA nucleotide sequences were classified
into 28 and 26 haplotypes, respectively. In phylogenetic trees, the haplotypes clustered
into 2 haplogroups corresponding to haplogroups A and B, which were previously reported.
Haplogroups A and B were further subdivided into sub-haplogroups AJ1 and AJ2, and BJ1 and
BJ2, respectively. In both trees, the sequences of haplotypes in AJ1 and BJ2 were
relatively distant from those reported in other countries, while some sequences in AJ2 and
BJ1 were identical to those in Europe. In addition, the ITS sequences were classified into
two sequences, and both sequences were closely related to the sequences found in European
countries. These findings indicate a possibility of international oversea transmission of
D. gallinae. |
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Keywords: | 16S rRNA gene COI gene ITS region Japan phylogenetic diversity poultry red mite |
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