Field performance of highbush blueberries (Vaccinium × corymbosum L.) cv. ‘Herbert’ propagated by cuttings and tissue culture |
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| Institution: | 1. Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstraße 1, 38106, Braunschweig, Germany;2. Institute of Horticultural Production Systems, Woody Plant and Propagation Physiology Section, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hanover, Germany;1. Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas LT-50254, Lithuania;2. Kaunas Botanical Garden of Vytautas Magnus University, Žilibero g. 6, Kaunas LT-46324, Lithuania;1. Cátedra de Fruticultura, Facultad de Ciencias Agrarias, Universidad Nacional de Lomas de Zamora e Instituto de Investigación sobre Producción Agropecuaria, Ambiente y Salud (IIPAAS-CIC), Ruta 4, Km. 2, (1836) Llavallol, provincia de Buenos Aires, Argentina;2. CERZOS-Departamento de Agronomía, Universidad Nacional del Sur, Bahía Blanca, provincia de Buenos Aires, Argentina;3. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA/CONICET-UBA), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina;1. Plant Breeding and Phenomics Center, Faculty of Agricultural Sciences, Universidad de Talca, 2 Norte 685, Talca, Chile;2. Centro de Investigación y Transferencia en Riego y Agroclimatología (CITRA), Universidad de Talca, Chile;3. Instituto de Ciencias Biológicas, Universidad de Talca, Talca, Chile;1. Departamento de Ciencias Agronómicas y Recursos Naturales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54 D, Temuco, Chile;2. Centro de Fruticultura, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54 D, Temuco, Chile;3. Center of Applied Ecology and Sustainability (CAPES), Santiago, Chile;4. Center for Sustainability Research, Universidad Andres Bello, Santiago, Chile |
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| Abstract: | ‘Herbert’ highbush blueberry (Vaccinium × corymbosum L.) plants propagated by softwood cuttings (HT) and obtained by micropropagation (TC) of axillary (AX) and adventitious (AD) shoots of 1-year-old in vitro cultures or 11-year-old cultures (SH) were compared. Propagation methods exerted significant influence on nursery and field performance of blueberries. Cutting-derived HT plants grew more slowly, produced significantly less and shorter shoots and were more variable than micropropagated plants. However, the majority of HT plants developed flowers 1 year earlier, flowered more abundantly, bore significantly larger berries than TC plants. There was no clear difference between AX and AD plants. SH-derived plants had smaller berries with the fewest seeds compared to AX and AD-obtained plants. This reveals that culture age had more significant influence than shoot source for the variation observed among micropropagation systems. The present study underlines the necessity of frequent establishment of in vitro cultures of highbush blueberry and carry them out by limited number of passages. |
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