| 木本菊的组织培养及快繁技术研究 |
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| 引用本文： | 杨玉田,朱宗贵,刘富先.木本菊的组织培养及快繁技术研究[J].安徽农业科学,2007,35(19):5740-5740,5774. |
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| 作者姓名： | 杨玉田 朱宗贵 刘富先 |
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| 作者单位： | 山东省枣庄市农科院,山东枣庄,277322;山东省枣庄市农科院,山东枣庄,277322;山东省枣庄市农科院,山东枣庄,277322 |
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| 摘 要： | 以木本菊茎段为外植体,研究了木本菊的组织培养及快繁技术。结果表明,最佳芽分化培养基是MS+1.0 mg/L 6-BA+0.1 mg/LIAA+1.0%糖,约60~70 d可诱导出丛生芽;在MS+0.5 mg/L 6-BA+0.2 mg/L NAA+1.0%糖的培养基上增殖速度最快,每升10 g的蔗糖浓度也最有利于芽的增殖;最适的扎根培养基是1/2 MS+0.1~0.3 mg/L NAA+1.0%糖或1/2 MS+0.05 mg/L 6-BA+0.5 mg/L NAA+1.0%糖,6~10 d可扎根,35~40 d可移栽。
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| 关 键 词： | 木本菊 组织培养 快繁技术 |
| 文章编号： | 0517-6611（2007）19-05740-01 |
| 修稿时间： | 2007-03-01 |
Studies on Tissue Culture and Rapid Propagation Technique in Woody Chrysanthemum |
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| YANG Yu-tian et al;.Studies on Tissue Culture and Rapid Propagation Technique in Woody Chrysanthemum[J].Journal of Anhui Agricultural Sciences,2007,35(19):5740-5740,5774. |
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| Authors: | YANG Yu-tian ; |
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| Institution: | Zaozhuang Academy of Agriculture Science of Shandong Province; Zaozhuang; Shandong 277322 |
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| Abstract: | The lab test with stem segment of woody chrysanthemum as explant showed that the optimum bud differentiation medium was MS+1 mg/L 6-BA+0.1 mg/L IAA+1.0% sugar,which induced the cluster bud after culturing for 60～70 days.The multiplication speed on the medium of MS+0.5 mg/L 6-BA+0.2 mg/L NAA+1.0% sugar was the highest.The cane sugar concentration 10.0 g/L was most in favor of bud multiplication.The optimum rooting medium was 1/2 MS+0.1～0.3 mg/L NAA+1.0% sugar or 1/2 MS+0.05 mg/L 6-BA+0.5 mg/L NAA+1.0% sugar,which could take the root at 6～10 days and transplanted at 35～40 days. |
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| Keywords: | Woody chrysanthemum Tissue culture Rapid propagation |
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