暗褐网柄牛肝菌优良菌株的筛选

以8个不同采集地来源的暗褐网柄牛肝菌(Phlebopus portentosus)菌株为试验材料,分别进行了菌丝固体培养、液体培养初筛和出菇复筛试验。结果表明:8个供试菌株在M1琼脂培养基(200g马铃薯、20g葡萄糖、2g酵母膏、1g MgSO_4、1g KH_2PO_4、20g琼脂、1000mL水)上菌丝长势均粗壮浓密,在M1液体培养基(M1琼脂培养基不加琼脂)中,除菌株Cy3和Cy6(因菌丝生长缓慢或几乎不继续生长而被淘汰)外其它6个菌株均生长良好;出菇试验中,菌株Cy4表现优良栽培性状:菌丝在培养料中长势强,子实体产量高单菇重(101.0±5.2)g,成熟率95%],商品性状好(菌盖黑褐色、菌柄黄色粗壮和基部稍膨大)。

Isolation and Screening of High QualityPhlebopus portentosus Strains

Mycelia were isolated from eight Phlebopus portentosus fruit bodies collected from the wild at different locations in Yunnan and Sichuan Provinces.Mycelial growth rates on M1 medium, consisting of 20.0 g glucose, 1.0 g MgSO4,1.0 g KH2PO4, 2.0 g yeast extract and 200 g potato in 1000 mL H2O, were then used to select six isolates (Cy1,Cy2,Cy4,Cy5,Cy7 and Cy8) for fruiting tests.Mycelial growth on M1 agar medium (M1 medium + 20 g agar), and mycelial growth and fruiting characteristics of the isolates when cultivated on a substrate consisting of 62.8% rubber tree sawdust,11% garden or orchard soil,25% Olyza sativa,1% CaCO3,0.1% MgSO4 and 0.1% KH2PO4 were then determined in order to screen for high quality strains suitable for commercial cultivation.Vigorous mycelial growth, the shortest time required to fully colonize the cultivation substrate (~21 d), the highest average fruit body weight (101.0±5.2) g], and the highest percentage of bags containing fruit bodies with pileus diameter ≥4 cm (95%), were recorded for isolate Cy4, collected from a location in Xinping Town, Menghai County, Yunnan Province.Fruit bodies of this isolate also exhibited excellent characteristics consisting of a thick stipe with a slightly swollen base and a dark brown cap.