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| 抗真菌乳酸菌的筛选及菌种鉴定 | |
| 引用本文： | 吕欣,高宇,张晓娟,董明盛,陈晓红,姜梅.抗真菌乳酸菌的筛选及菌种鉴定[J].中国农学通报,2006,22(3):98-101. |
| 作者姓名： | 吕欣 高宇 张晓娟 董明盛 陈晓红 姜梅 |
| 作者单位： | 1. 南京农业大学食品科技学院,江苏,南京,210095 2. 南京理工大学科技处,江苏,南京,210096 3. 总后勤部军需装备研究所,北京,100010 |
| 基金项目： | 南京农业大学校科研和教改项目 |
| 摘 要： | 采用双层培养基法从中国传统发酵食品(发酵乳、泡菜、发酵肉)中筛选到20株对真菌有拮抗作用的乳酸菌菌株。对其中7株具有明显拮抗作用菌株的抑菌谱进行了初步研究,这7株菌对啤酒酵母、汉逊酵母、枯草芽胞杆菌等有明显的抑制作用,对黑曲霉可明显抑制其孢子的成熟。对其中2株菌进行了常规生理生化及16SrRNA分子鉴定,鉴定结果为干酪乳杆菌鼠李糖亚种。 |
| 关 键 词： | 抗真菌 乳酸菌 生物防腐 鉴定 |
| 收稿时间： | 2006-02-05 |
| 修稿时间： | 2006-02-052006-02-10 |
The Screening and Identification of Antifungal Lactic Acid Bacteria |
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| Lu Xin,Gao Yu,Zhang Xiaojuan,Dong Mingsheng,Chen Xiaohong,Jiang Mei.The Screening and Identification of Antifungal Lactic Acid Bacteria[J].Chinese Agricultural Science Bulletin,2006,22(3):98-101. | |
| Authors: | Lu Xin Gao Yu Zhang Xiaojuan Dong Mingsheng Chen Xiaohong Jiang Mei |
| Institution: | 1.The college of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095; 2.Nanjing University of Science and Technology, Nanjing 210096; 3.The quartermaster equipment institue of the GLD of PLA, Beijing 100010 |
| Abstract: | Twenty antifungal lactic acid bacteria strains were screened by di-layer medium method from traditional Chinese fermented food(tibet kefir, pickled vegetables, fermented meat). The antifungal and antibacterial spectrum was studied, it was found that seven strains have obvious antagonistic effect on Saccharomyces cerevisiae, Debaryomyces hansenii, Bacillus subtilis, etc. and they can also inhibit the spore maturation of Aspergillus niger. Then the traditional identification and 16S rRNA molecular identification were done for two strains, the result showed that they were Lactobacillus casei ssp. Rhamnosus. |
| Keywords: | Anti-fungal Lacic acid bacteria Biopreservation Identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |