提高鸡传染性支气管炎病毒血凝抗原滴度途径及抗原灭活

将７株传染性支气管炎病毒（ＩＢＶ）标准株（Ｍ４１、Ｈ１２０、Ｈｏｌｔｅ、Ｇｒａｙ、Ｃｏｎｎｅｃｔｉｃｕｔ、Ｉｏｗａ６０９和Ｔ株）和６株分离株（ＮＩＢＶ、ＧＩＢＶ、Ｍ、ＳＨ、Ｊ和Ｈ株）分别接种于鸡胚，收获尿囊液，经浓缩后，用魏氏梭菌培养液处理，制备血凝抗原。其中，Ｈ１２０株血凝滴度最高，Ｔ、Ｍ、Ｊ和Ｈ株无血凝性。应用含有不同滴度ＩＢＶ母源抗体的鸡胚增殖病毒制备抗原，效价与用ＳＰＦ鸡胚增殖病毒制备的抗原效价一致。尿囊液经反复冻融后再制备抗原会使血凝价降低。抗原分别用甲醛、高碘酸钠、硼氢化钾和ＳＤＳ灭活，其中甲醛灭活效果最理想。抗原对氯仿敏感，对乙醚稳定。适宜浓度的Ｎａ＋、Ｍｇ２＋可显著提高抗原的血凝性

The Ways to Improve the Hemagglutination Antigen Titers of the Avain Infectious Bronchitis Virus and Its Inactivation

Seven reference of avain infectious bronchitis virus (IBV) (M 41 , H 120 , Holte, Gray, Connecticut, Iowa609 and T strains) and six isolates (NIBV, GIBV, M, H, J, and H strains) were inoculated in chicken embryos to prepare the hemagglutination(HA) antigen. The allantoic fluid (AAF) was harvested and concentrated, then treated with the culture of clostridium welchii. Among these strains, only H 120 strain revealed the highest HA titer, whilst the T, M J and H strains did not at all. The viruses were propagated in the chicken embryos with different HI titers of maternal antibody, and there was no difference among these embryos in the HA titers. After repeated freezing thawing of the viruses, the HA titers reduced obviously. When the antigens were inactivated with formalin, Sodium periodate, postassium borohydride and SDS, formalin was shown to be the best for inactivation and the antigens sensitive to chloroform, but tolerant toward the others. An appropriate concentration of Na or Mg 2 could improve the HA activity markedly.