| 花椒愈伤组织EMS诱变及变异研究 |
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| 引用本文： | 薛惠丹,李周岐.花椒愈伤组织EMS诱变及变异研究[J].西北林学院学报,2012(2):98-101. |
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| 作者姓名： | 薛惠丹 李周岐 |
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| 作者单位： | 西北农林科技大学林学院 |
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| 基金项目： | 陕西省“13115”科技创新工程项目(2007ZDKG-12);西北农林科技大学唐仲英育种基金项目 |
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| 摘 要： | 用EMS处理花椒韧皮部及叶片来源的愈伤组织,确定愈伤组织的适宜来源和EMS处理花椒愈伤组织LD50,在不同EMS浓度的半致死剂量下处理愈伤组织,组培获得花椒诱变植株。结果表明,EMS诱变花椒愈伤组织的LD50为0.3%EMS处理6h,0.5%EMS处理4h,0.7%EMS处理2h。诱变后愈伤组织经增殖和分化形成完整植株。通过对比再生植株形态学差异,筛选出变异植株340株。
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| 关 键 词： | 花椒 愈伤组织 EMS 变异 |
Variation and EMS Mutagenesis in Callus of Zanthoxylum bungeanum |
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| XUE Hui-dan,LI Zhou-qi.Variation and EMS Mutagenesis in Callus of Zanthoxylum bungeanum[J].Journal of Northwest Forestry University,2012(2):98-101. |
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| Authors: | XUE Hui-dan LI Zhou-qi |
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| Institution: | (College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The callus of phloem and leaves of Zanthoxylum bungeanum were treated by ethyl methane sulfonate(EMS) to determine the semi-lethal concentration of EMS and to get suitable source of callus.The results showed that the semi-lethal concentrations of EMS were 0.3%(with 6 h treatment),0.5%(4h),0.7%(2h).From the regeneration plants which had been mutated,we found numerous variations such as the length and width of the leaves.Through examining the morphological characteristics,340 mutation plants were selected. |
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| Keywords: | Zanthoxylum bungeanum callus ethyl methane sulfonate(EMS) variation |
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