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3种测定大豆种子脂肪含量方法的比较研究
引用本文:马爱萍,邵群,孙志宾,程文,李继存. 3种测定大豆种子脂肪含量方法的比较研究[J]. 河南农业科学, 2011, 40(3): 62-64
作者姓名:马爱萍  邵群  孙志宾  程文  李继存
作者单位:1. 山东师范大学,生命科学学院,山东省逆境植物重点实验室,山东,济南,250014
2. 济宁市农业科学院经济作物所,山东,济宁,272031
基金项目:国家转基因重大专项,山东省自然科学杰出青年基金项目
摘    要:
快速无损检测大豆种子中的脂肪含量,在大豆品质育种和质量检测上至关重要.分别用索氏提取法、核磁共振仪及近红外谷物分析仪测定了5种国产大豆种子的脂肪含量,并对3种方法的测量结果进行了分析比较.结果表明:核磁共振法测定的结果与索式提取法更接近,偏差均小于0.95个百分点,最低偏差仅为-0.02个百分点;而近红外光谱法与索式提...

关 键 词:大豆  脂肪含量  索氏提取法  核磁共振法  近红外光谱法

Comparison of Three Methods for Fat Content Measurement in Soybean Seeds
MA Ai-ping,SHAO Qun,SUN Zhi-bin,CHENG Wen,LI Ji-cun. Comparison of Three Methods for Fat Content Measurement in Soybean Seeds[J]. Journal of Henan Agricultural Sciences, 2011, 40(3): 62-64
Authors:MA Ai-ping  SHAO Qun  SUN Zhi-bin  CHENG Wen  LI Ji-cun
Affiliation:1.Key Laboratory of Plant Stress Research of Shandong Province,College of Life Science,Shandong Normal University,Jinan 250014,China;2.Economic Plants Institute,Jining Academy of Agricultural Sciences,Jining 272031,China)
Abstract:
The fat contents of five domestic soybean cultivars seeds were analyzed by Soxhlet method,nuclear magnetic resonance analyzer(NMR) and near-infrared(NIR) grain analyzer respectively.The results showed that results of NMR closely tallied with that of Soxhlet method,the deviation between them was less than 0.95 percentage points,and the minimum deviation was only-0.02 percentage points.However,the result of NIR showed higher deviation from that of Soxhlet method than NMR did in this study.
Keywords:Soybean  Fat content  Soxhlet method  Nuclear magnetic resonance  Near infrared spectroscopy
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