胚胎移植受体山羊同期发情方案的研究

对山羊进行3种同期发情处理,处理Ⅰ为C IDRS+PMSG(100 U)+PG(0.1 mg),处理Ⅱ为C IDRS+PMSG(200 U)+PG(0.1 mg),处理Ⅲ为PG(0.1 mg)。结果发现,撤栓后0~24 h处理Ⅰ的发情率(27.76±0.62)%与处理Ⅱ的发情率(35.00±5.00)%差异不显著(P>0.05),但均显著高于处理Ⅲ(3.62±0.12)%(P<0.05);撤栓后24~48 h和48~72 h处理Ⅰ(35.31±1.40)%和(5.86±0.73)%、处理Ⅱ(56.67±3.33)%和0%和处理Ⅲ(18.07±1.94)%和(14.45±1.94)%的发情率之间均差异显著(P<0.05);在撤栓后0~72 h,处理Ⅰ(68.05±1.66)%和处理Ⅱ(91.67±8.33%)的发情率均显著高于处理Ⅲ(36.14±3.88)%P<0.05,但是处理Ⅰ、Ⅱ之间显著不差异(P>0.05)。另外在手术中观察山羊卵巢发现,处理Ⅰ、Ⅱ和Ⅲ有较好黄体的山羊比率分别为(52.15±1.42)%、(46.67±3.33)%和(62.88±2.43)%,处理Ⅰ和Ⅲ高于处理Ⅱ,并且处理Ⅱ和处理Ⅲ之间差异显著(P<0.05)。试验结果表明,采用2次优化PG法既可提高发情率,又可使受体羊黄体率达到较好水平。

Optimization of goats synchronization estrus and transplantation of the transgenic embryo

In the present experiment,suppository sponge+ PSMG(100 U)+ PG(0.1 mg)(treatmentⅠ),progesterone suppository sponge+ PMSG(200 U)+ PG(0.1 mg)(treatmentⅡ) and PG(0.1 mg)(treatmentⅢ) were used in goat synchronization of estrus.The results demonstrated that no significant differences between treatmentⅠ(27.76±0.62)% and treatmentⅡ(35.00±5.00)% in estrus rate after removing pessulum 0~24 h(P>0.05),but significantly higher than that of treatment Ⅲ(3.62±0.12)%(P<0.05).There were significant differences among treatmentⅠ(35.31±1.40)% and(5.86±0.73)%,treatmentⅡ(56.67±3.33)% and 0% and treatment Ⅲ(18.07±1.94)% and(14.45±1.94)% after removing pessulum 24~48 h and 48~72 h(P<0.05).In addition,the estrus rates of treatmentⅠ(68.05±1.66)% and treatmentⅡ(91.67±8.33)% were significantly higher than that of treatment Ⅲ(36.14±3.88)%(P<0.05),but no significant differences between treatment Ⅰand treatmentⅡ(P>0.05) in 0~72 h.By observing the ovaries of goats,we found the rate of good quality corpus luteum in treatmentⅠ,treatmentⅡ and treatmentⅢ was(52.15±1.42)%,(46.67±3.33)% and(62.88±2.43)%,respectively.Together,treatment Ⅲ showed more effective than treatmentⅡ(P<0.05).