| 迟缓爱德华菌人工感染斑马鱼的致病性及组织病理学变化 |
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| 引用本文： | 彭国瑞,王成龙,王雪鹏,陆承平.迟缓爱德华菌人工感染斑马鱼的致病性及组织病理学变化[J].畜牧与兽医,2011,43(1). |
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| 作者姓名： | 彭国瑞 王成龙 王雪鹏 陆承平 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏,南京,210095
2. 山东农业大学动物科技学院,山东,泰安,271018 |
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| 基金项目： | 国家大学生创新性实验计划项目，浙江省近岸水域生物资源开发与保护重点实验室开放课题基金，山东农业大学青年科技创新基金 |
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| 摘 要： | 用迟缓爱德华菌真鲷分离株(E.t-CD)对斑马鱼进行人工感染试验,观察其发病、死亡以及相关病理学变化。结果表明,该菌对斑马鱼的半数致死浓度(LD50)为2.69×102 cfu/尾;感染发病鱼呈现岀血、溃疡、腹水、败血等症状;病理组织学检查以肝脏水肿变性,肝细胞萎缩、坏死、脱落;脾脏散在增生性结节、充血、水肿、淋巴细胞大量缺失等病变为主。结果表明,斑马鱼可作为研究迟缓爱德华菌致病性的动物模型。
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| 关 键 词： | 迟缓爱德华菌 斑马鱼 致病性 病理学 |
The pathogenicity and histopathological changes of artificially infected zebrafish with Edwardsiella tarda |
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| PENG Guo-rui,WANG Cheng-long,WANG Xue-peng,LU Cheng-ping.The pathogenicity and histopathological changes of artificially infected zebrafish with Edwardsiella tarda[J].Animal Husbandry & Veterinary Medicine,2011,43(1). |
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| Authors: | PENG Guo-rui WANG Cheng-long WANG Xue-peng LU Cheng-ping |
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| Institution: | PENG Guo-rui1,WANG Cheng-long1,WANG Xue-peng1,2*,LU Cheng-ping1(1.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China,2.College of Animal Science & Technology,Shandong Agricultural University,Tai'an 271018,China) |
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| Abstract: | Clinical manifestations and pathological changes were observed after the zebrafish were artificially infected with Edwardsiella tarda strain CD(E.t-CD).The results showed that the median lethal dose(LD50) for zebrafish was 2.69×102 cfu/fish,and the infected fish showed bleeding,ulcers,ascites,sepsis and other symptoms.Histopathological observation showed that swelling degeneration,atrophy,necrosis and abscission in the liver cells;scattered hyperplastic nodules,congestion,edema and absence of lymphocyte in the spleen cells.The results indicated that the zebrafish could be used as the animal models for studying the pathogenicity of E.tarda. |
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| Keywords: | Edwardsiella tarda zebrafish pathogenicity pathology |
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