| 杂交狼尾草新品系遗传关系的RAPD分析 |
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| 引用本文： | 陈平,朱钧,刘萍,石秀兰.杂交狼尾草新品系遗传关系的RAPD分析[J].中国草地学报,2007,29(3):34-38. |
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| 作者姓名： | 陈平 朱钧 刘萍 石秀兰 |
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| 作者单位： | 1. 仲恺农业技术学院,广东,广州,510225
2. 仲恺农业技术学院,广东,广州,510225;西南大学园艺园林学院,重庆,400715 |
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| 基金项目： | 广东省良种培育和引进专项基金 |
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| 摘 要： | 利用RAPD分子标记技术分析狼尾草属11个品种（品系）12份材料间的遗传关系,结果发现7个引物共扩增出82条带,其中多态性条带为60条,多态位点达73.2%,表明在这11个品种（品系）间具有较高的遗传多样性。从SPSS聚类分析结果发现,杂交狼尾草新品系1号、杂交狼尾草新品系2号与原品种杂交狼尾草有较为密切的亲缘关系,杂交狼尾草新品系1号与杂交狼尾草的遗传距离为0.033,杂交狼尾草新品系2号与杂交狼尾草的遗传距离也仅为0.067,从而为杂交狼尾草新品系1号、杂交狼尾草新品系2号与杂交狼尾草之间亲缘关系的确定提供了重要依据。此外,也发现象草新品系与摩特矮象草具有最小的遗传距离（0.033）。
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| 关 键 词： | 狼尾草新品系 遗传多样性 |
| 文章编号： | 1673-5021（2007）03-0034-05 |
| 修稿时间： | 2006-11-032007-03-09 |
Analysis of Genetic Relationships in New Lines of Hybrid Pennisetum by RAPD Markers |
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| CHEN Ping,ZHU Jun,LIU Ping,SHI Xiu-lan.Analysis of Genetic Relationships in New Lines of Hybrid Pennisetum by RAPD Markers[J].Chinese Journal of Grassland,2007,29(3):34-38. |
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| Authors: | CHEN Ping ZHU Jun LIU Ping SHI Xiu-lan |
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| Institution: | 1. Zhongkai University of Agriculture and Technology, Guangzhou 510225, China; 2. College of Horticulture and Landscape, Southwest University, Chongqing 400716, China |
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| Abstract: | The genetic relationships of 11 cultivars and lines in Pennisetum were analyzed using random amplified polymorphic DNA(RAPD) markers.A total of 82 reproducible RAPD fragments were produced using 8 random primers.60 fragments(73.2% of the total observed) were polymorphic which indicated that there was high genetic diversity among these 11 genotypes.From the results of SPSS cluster analysis,the 'New line' No.1 of hybrid Pennisetum was most similar to Hybrid Pennisetum(genetic distance 0.033),the 'New line' No.2 of hybrid Pennisetum was similar to Hybrid Pennisetum(genetic distance 0.067),which indicated that RAPD molecular marker could be used for genetic relationship researches on Pennisetum.In addition,the 'New line' of elephantgrass was most similar to 'Mott'(genetic distance 0.033). |
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| Keywords: | RAPD |
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