| 鸭肝炎病毒Ⅰ型的RT-PCR诊断方法的建立 |
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| 引用本文： | 高骏,孙凤萍,胡建华,王胜昌,易建中. 鸭肝炎病毒Ⅰ型的RT-PCR诊断方法的建立[J]. 上海交通大学学报(农业科学版), 2008, 26(2): 124-126 |
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| 作者姓名： | 高骏 孙凤萍 胡建华 王胜昌 易建中 |
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| 作者单位： | 上海农业科学院畜牧兽医研究所,上海,201106;上海实验动物研究中心,上海,200032 |
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| 基金项目： | 上海市科委环境条件计划项目 |
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| 摘 要： | 根据Genebank上发表的鸭肝炎病毒Ⅰ型（DHV-1）的部分序列设计了1对引物D1／D2，通过对反应条件的优化，建立起了有效的鸭肝炎病毒Ⅰ型的RT—PCR诊断方法。并应用此方法对近年在上海及周遍地区分离到的2株DHV-1分离毒进行了检测和测序鉴定，结果证实此株分离毒为DHV-1病毒。结果显示建立的DHV—1的PER诊断方法具有快速、准确的优点。
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| 关 键 词： | 鸭肝炎病毒Ⅰ型 DHV-1 RT-PCR |
| 文章编号： | 1671-9964(2008)02-0124-03 |
| 修稿时间： | 2007-10-08 |
RT-PCR Method for Detection of Duck Hepatitis Virus I |
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| GAO Jun SUN Feng-ping HU Jian-hua WANG Sheng-chan YI Jian-zhong. RT-PCR Method for Detection of Duck Hepatitis Virus I[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2008, 26(2): 124-126 |
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| Authors: | GAO Jun SUN Feng-ping HU Jian-hua WANG Sheng-chan YI Jian-zhong |
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| Affiliation: | GAO Jun~1 SUN Feng-ping~1 HU Jian-hua~2 WANG Sheng-chan~2 YI Jian-zhong~1 (1.Animal Husb,ry , Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,Chian,2.Shanghai Laboratory Animals Research Centre,Shanghai 200032,Chian) |
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| Abstract: | We designed a pair of primers D1/D2 according to the sequences of DHV-1 strain registered by Genebank.We established and optimized the RT-PCR method to detect the DHV-1.The two Shanghai isolates of DHV-1 can be detected by the RT-PCR method.It was shown that the DHV-1 RNA of the isolates could be amplified by RT-PCR and we sequenced the products of RT- PCR to confirm the isolates are DHV-1.The results show that the established PCR method is a quick and exact method for DHV-1 detection. |
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| Keywords: | duck hepatitis virus I DHV-1 RT-PCR |
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