夏枯草(Prunella vulgaris)组织培养和快速繁殖

以夏枯草的叶片、茎节、顶芽为外植体在含不同浓度的6-BA和NAA的MS培养基中培养,比较其增值率和分化率.发现以茎节为外植体,诱导不定芽的最佳培养基为MS 3.0 mg/L 6-BA 0.1 mg/L NAA,分化率为100%,增殖倍数为6.8.以顶芽为外植体,不定芽的最佳培养基为MS 3.0 mg/L 6-BA 0.05 mgm NAA或MS 3.0 mg/L 6-BA 0.1 mg/L NAA,分化率分别为88.2%和84.6%,增殖倍数分别为5.8和6.2.最佳生根培养基为1/4MS NAA 0.5 mg/L,生根率为100%.将生根的组培苗经过驯化移栽后,成活率为93%.表明,叶片难以分化;茎节的分化率效果最好;顶芽的分化能力介于二者之间.

Tissue Culture and Rapid Propagation of Prunella vulgaris

In this study, leaves, stem internodes and apical bud of Prunella vulgaris used as the explants were placed on MS medium supplemented with different concentration 6-BA and NAA and then compared their rate of proliferation and differentiation. The best medium and hormone combination for apical bud and stem intemodes was established. MS medium containing 3.0 mg/L 6-BA and 0.1 mg/L NAA suited proliferation culture for stem internode. The rate of differentiation was 100%, and the proliferation multiple was 6.8. MS medium containing 3.0 mg/L 6-BA and 0.05 mg/L NAA or MS medium containing 3.0 mg/L 6-BA and 0.1 mg/L NAA suited differentiation or proliferation culture for apical bud. The rate of differentiation was 88.2% and 84.6%, and the proliferation multiple was 5.8 and 6.2 separately. The best rooting medium was 1/4 MS medium with 0.5 mg/L NAA. The rooting rate was 100%. The seedlings with roots were transplanted after domestication. The surviving rate was 93%. The result showed that the different explants had differentiation capacity. The leaves were difficult to be induced and stem internode was easy to be induced.