一组高效棉秆降解菌复合系的构建及其降解特性研究

根据微生物之间协同作用的原理,采用多代淘汰及不同系之间组配的方法,筛选构建了一组高效棉秆降解菌复合系--ZFC菌系.在不同培养条件下对ZFC菌系进行液体发酵,研究比较了发酵过程中的pH、棉秆失重率、菌体生长、纤维素酶活力等指标.结果表明:28℃静置液体培养条件更适合ZFC菌系的生长产酶和对棉秆的降解.在28℃静置培养条件下经过13 d的液体发酵,ZFC菌系对棉秆中纤维素、半纤维素和木质素的降解率分别为21.0;、65.1;和46.3;,说明研究所构建的一组棉秆降解菌复合系--ZFC菌系可加速棉秆的降解.

Construction of a Composite Microbial System with High Efficient Cotton Stalk Degradation and Its Degradation Characteristics

According to the principle of synergistic action of different microorganisms,a composite microbial system ZFC with high efficient cotton stalk degradation was screened and constructed through the methods of multigeneration selection and combination of different microbial communities.Liquid fermentation of the microbial system ZFC was conducted under different culture conditions.The pH,weight loss rate of cotton stalks,microbial growth and cellulase activity in the process of fermentation were studied and compared.The results showed that the condition of static culture at 28℃ was more suitable for growth,enzyme production and cotton stalk degradation of the microbial system ZFC.The degradation rate of cellulose,hemicellulose and lignin in cotton stalk by the microbial system ZFC were 21.0%,65.1% and 46.3%,respectively,for 13 d under the condition of static culture at 28℃,which indicated that the composite microbial system ZFC constructed by this study can accelerate the degradation of cotton stalk.