In vitro somatic embryogenesis from Mangifera indica L. callus |
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Authors: | Richard E Litz Robert J Knight Shmuel Gazit |
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Institution: | 1. University of Florida, Agricultural Research and Education Center, Institute of Food and Agricultural Sciences, 18905 SW 280 Street, Homestead, FL 33031 U.S.A.;2. United States Department of Agriculture, Subtropical Horticulture Research Unit, Miami, FL 33158 U.S.A.;3. Department of Horticulture, Volcani Institute, Bet Dagan Israel |
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Abstract: | The nucellus and globular adventitious proembryos were removed from 2-month-old fruits of mango (Mangifera indica L.) cultivars ‘Ono’ and ‘Chino’, and were cultured on sterile, solid Murashige and Skoog (MS) medium that had been modified as follows: half-strength major salts and chelated iron; 20% (v/v) coconut water (CW); 6% sucrose; 100 mg l?1 ascorbic acid and 400 mg l?1 glutamine. Embryogenic explants were sub-cultured after 4–6 weeks in liquid modified MS medium containing 2 mg l?1 2,4-dichlorophenoxyacetic acid (2,4-D) instead of CW. Rapidly growing cultures were established and were sub-cultured monthly. Somatic embryogenesis was induced following sub-culture from MS medium with 2,4-D to MS without growth regulators and with or without activated charcoal (0.5%). Germination of somatic embryos appeared to be enhanced by 1 mg l?1 benzyladenine (BA); however, most of the germinating embryos became embryogenic. |
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Keywords: | mango somatic embryogenesis tissue culture |
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