|
|||||
|
|
| 应用PCR-DHPLC技术高通量快速检测转基因玉米 | |
| 引用本文： | 吴静,李铁柱,孙瑶,孙方达,栾凤侠,赵明超.应用PCR-DHPLC技术高通量快速检测转基因玉米[J].玉米科学,2012,20(5):40-44. |
| 作者姓名： | 吴静 李铁柱 孙瑶 孙方达 栾凤侠 赵明超 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨,150030 2. 黑龙江出入境检验检疫局,哈尔滨,150001 3. 东北农业大学农学院,哈尔滨150030;黑龙江出入境检验检疫局,哈尔滨150001 |
| 基金项目： | 国家质检总局项目(2011IK200) |
| 摘 要： | 利用多重PCR结合DHPLC技术首次建立转基因玉米高通量快速检测方法。根据公布的转基因玉米外源基因序列设计并合成适合多重PCR的引物序列,以转基因玉米为模板,通过优化多重PCR和DHPLC的反应条件,建立一套同时快速筛选检测玉米中多种转基因成分的7重PCR-DHPLC检测方法。该方法的检测灵敏度为0.195 ng/μL,质粒检测灵敏度为1×103拷贝/μL。 |
| 关 键 词： | 转基因玉米 多重PCR技术 DHPLC技术 |
High-throughput and Rapid Detection of Genetically Modified Corn by PCR-DHPLC Technology |
|
| WU Jing , LI Tie-zhu , SUN Yao , SUN Fang-da , LUAN Feng-xia , ZHAO Ming-chao.High-throughput and Rapid Detection of Genetically Modified Corn by PCR-DHPLC Technology[J].Journal of Maize Sciences,2012,20(5):40-44. | |
| Authors: | WU Jing LI Tie-zhu SUN Yao SUN Fang-da LUAN Feng-xia ZHAO Ming-chao |
| Institution: | 2,et al.(1.Northeast Agricultural University,Harbin 150030;2.Heilongjiang Entry-Exit Inspection and Quarantine Bureau,Harbin 150001,China) |
| Abstract: | Established a method to detect transgenic corn by using the technology of Multiplex PCR with DHPLC rapidly and high-through for the first time.Multiplex PCR primers were designed and synthesized based on published transgenic corn exogenous gene sequences.To transgenic corn as a template,and established a seventh PCR-DHPLC detection method to screening a variety of genetically modified in corn simultaneously and rapidly by optimizing the multiplex PCR and DHPLC reaction conditions.The detection sensitivity of the method is 0.195 ng/μL,and the detection sensitivity of plasmid is 1 ×103copy/μL. |
| Keywords: | Genetically modified corn Multiplex PCR DHPLC technology |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |