Cytoplasmic relatedness of apple landraces and cultivars: a molecular analysis |
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| Authors: | S. Kato S. Ishikawa S. Imakawa S. Komori T. Mikami Y. Shimamoto |
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| Affiliation: | (1) Laboratory of Plant Genetics and Evolution, Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan;(2) Experiment Farms, Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan;(3) Morioka Branch, Fruit Tree Research Station, 020-01 Morioka, Japan;(4) Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan |
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| Abstract: | Summary Mitochondrial DNA (mtDNA) diversity has been determined in apple landraces and cultivars, based upon restriction fragment length polymorphisms (RFLPs) for several mitochondrial genes. Our analysis includes three cultivars,  Golden Delicious , McIntosh and Delicious, which represent the various patterns of mtDNA polymorphisms previously described (Ishikawa et al., 1992). A total of five RFLPs were detected, allowing classification of the apple genotypes into four cytoplasmic groups: GroupI, Golden Delicious-type; GroupII, McIntosh-type; GroupIII, Delicious-type; and Group IV, Dolgo Crab-type. European landraces and cultivars were assigned to Groups I, II, and III, while chinese crab apples were placed in either Group III or IV. |
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| Keywords: | apple cytoplasmic grouping diversity Malus x domestica mitochondrial DNA RFLPs |
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