地方品种鸡ev21基因和JS序列分布对鸡羽速表型的影响

试验旨在探究多个地方品种快慢羽鸡的内源性病毒基因21(ev21)以及SPEF2基因与PRLR基因的部分重复序列(JS序列)分布对羽速基因(Kk)表型的影响。采用PCR扩增、HaeⅢ限制性内切酶酶切的方法检测不同地方品种快慢羽鸡性染色体上OR区域(ev21占据片段)、UR区域(URa、URb(ev21未占据片段))以及JS序列分布情况。结果表明:国内的一些地方品种部分慢羽鸡个体OR区域ev21基因缺失以及部分快羽鸡的URb区域存在ev21基因插入;可通过JS序列扩增对快慢羽表型进行准确鉴定。研究结果显示JS序列可作为快慢羽鉴定候选基因,该方法可广泛应用于国内快慢羽鸡种的鉴定。

Effect of ev21 Gene and JS Sequence of Distribution Local Breed Chickens on Feather Speed Phenotype

The aim of the experiment was to investigate the effects of endogenous viral gene(ev21) and the junctionsite of SPEF2 gene and PRLR gene sequences(JS sequence) on the phenotype of feather speed gene(Kk) in severaldomestic local breeds. The PCR amplification and Hae Ⅲ restriction endonuclease digestion were used in this experiment.The distribution of OR region(ev21 occupied region), UR region(URa, URb(ev21 unoccupied region)) and JS sequencedistribution on the sex chromosomes of early-feathering and late-feathering of different local breeds were detected. Theresults showed that there were ev21 gene deletions in some late-feathering individuals and ev21 gene insertions insome URb regions of domestic chicken breeds. Fast and slow feather phenotypes could be identificated accurately byJS sequence amplification. It was concluded that the JS sequence could be used as a candidate gene for early andlate-feathering identification. This method can be widely used in the identification of domestic early and late-featheringchicken breeds.