Genetic, phenotypic and pathogenic diversity among xanthomonads isolated from pistachio (Pistacia vera) in Australia |
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| Authors: | A Marefat E S Scott K Ophel-Keller M Sedgley |
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| Institution: | School of Agriculture and Wine, University of Adelaide, Waite Campus, PMB 1, Glen Osmond, South Australia 5064;;South Australian Research and Development Institute, GPO Box 397, Adelaide, South Australia 5001;and;Faculty of the Sciences, University of New England, Armidale, New South Wales 2351, Australia |
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| Abstract: | Repetitive extragenic palindromic polymerase chain reaction (rep-PCR), sequencing of the 16S?23S rDNA internal transcribed spacer (ITS), biochemical and physiological tests, the Biolog microplate system, polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins, and pathogenicity tests were used to characterize variability among xanthomonads isolated from pistachio trees suffering from bacterial dieback in four regions of Australia. ITS sequencing and rep-PCR revealed two distinct genotypes among the strains. The ITS sequencing suggested that the pistachio strains were closely related to Xanthomonas translucens pathovars, in particular X. translucens pv . poae . Results of physiological and biochemical tests, as well as Biolog microplate analysis and protein profiling, confirmed the existence of two groups. Furthermore, pathogenicity and host-range studies indicated that the two groups were biologically different. There was an association between the two groups and the geographical origin of the strains. |
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| Keywords: | 16S?23S rDNA sequencing Biolog microplate pathovars repetitive PCR SDS–PAGE Xanthomonas translucens |
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