| Abstract: | Objective]To observe the protective effects and pathway of paeoniflorin on rat MID(multi-infarct dementia) model by establishing the improved MID model,and to lay the pharmacological basis for the development of protective neurons candidate compounds. Methods]The classic rats' MID model was used to observe the protective effects and pathway of different dosages of paeoniflorin. The learning and memory abilities of rats were detected by Morris water maze. The expression of Bcl-2 and Bax protein were detected by immunohistochemistry.The changes of cerebral cortex and the neuron structure were detected by HE staining and electron microscopy. Results]Paeoniflorin had significant improvement effects on the time of anesthetic awareness,detention time of inclined plate test,behavioral evaluation,cognitive impairment and brain neuron injury of rat MID model. The protective mechanism of paeoniflorin was related to the Bcl-2 and Bax expression in cerebral cortex and hippocampus brain tissue. Conclusions]The paeoniflorin had significant improvement effects on the rat MID model. It restricted the EAA damage,intracellular calcium overload,and Bax expression,so as to promote the Bcl-2 expression and to restrict the cell apoptosis. |
