| 金线莲茎腐病病原菌分离及分子鉴定 |
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| 引用本文： | 赵云青,陈菁瑛,林晓军,刘保财,黄颖桢.金线莲茎腐病病原菌分离及分子鉴定[J].福建农业学报,2014(10):995-999. |
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| 作者姓名： | 赵云青 陈菁瑛 林晓军 刘保财 黄颖桢 |
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| 作者单位： | 福建省农业科学院农业生物资源研究所，福建 福州 350003; 福建省农业科学院药用植物研究中心，福建 福州 350003 |
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| 基金项目： | 公益性行业(农业)科研专项(201303117)；福建省农业科学院青年科技人才创新基金(2011QA 6)；福建省种业创新与产业化工程(2011FJZY)；福建省农业科学院农产品质量安全风险评估基金项目 |
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| 摘 要： | 采用组织块法对金线莲疑似茎腐病病原菌进行分离，用致病性试验、病原菌形态学观察、分子生物学方法对该病原菌进行鉴定。结果表明：从收集的金线莲疑似茎腐病病原菌感染植株中分离得到的菌株，在培养基中产生大量气生菌丝呈棉絮状，菌丝在培养基中产生紫色色素，有隔及分枝，大型分生孢子纺锤形，小型分生孢子卵形或长圆形。分离的菌株回接后可使金线莲植株产生病害，对扩增得到的rDNA-ITS序列进行 Blast对比分析，确定该病害为尖孢镰刀菌FusariumoxysporumSchl．引起的茎腐病。
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| 关 键 词： | 金线莲 茎腐病 病原菌 鉴定 |
Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii (Wall.)Lindl. |
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| ZHAO Yun-qing,CHEN Jing-ying,LIN Xiao-jun,LIU Bao-cai,HUANG Ying-zhen.Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii (Wall.)Lindl.[J].Fujian Journal of Agricultural Sciences,2014(10):995-999. |
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| Authors: | ZHAO Yun-qing CHEN Jing-ying LIN Xiao-jun LIU Bao-cai HUANG Ying-zhen |
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| Institution: | ZHAO Yun-qing, CHEN Jing-ying, LIN Xiao-jun, LIU Bao-cai, HUANG Ying-zhen (1. Institute of Agrobiological resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; 2. Medicinal Plant Research Center, Fujian academy of Agricultural Science, Fuzhou, Fujian 350003, China; 3. Fujian Industry of Chinese Medicinal Materials Association, Fuzhou, Fujian 350003, China) |
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| Abstract: | A suspected pathogen that caused the stalk rot on Anoectochilus roxburghii was isolated by means oftissue separation.It was subsequently subj ected to the pathogenicity tests,morphological observation and molecularbiology analysis for positive identification.The massive amount of aerial mycelia of the pathogen were gossypine,separate,branched,and capable of generating a purple pigment.Its macroconidium appeared in the shape of aspindle,and the microconidium in the shape of an egg.By inoculating the isolated strain to A .roxburghii ,apathogenicity to the plant was observed.Based on a blast test of rDNA-ITS sequence,the blight pathogen wasidentified to be Fusarium oxys porum Schl. |
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| Keywords: | Anoectochilus roxburghii (Wall )Lindl stalk rot pathogens identification |
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