| 漳州琯溪蜜柚果实黑斑病菌的分子鉴定 |
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| 引用本文： | 林石明,廖富荣,方志鹏,黄蓬英,陈红运,陈青,吴媛.漳州琯溪蜜柚果实黑斑病菌的分子鉴定[J].植物检疫,2010(5). |
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| 作者姓名： | 林石明 廖富荣 方志鹏 黄蓬英 陈红运 陈青 吴媛 |
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| 作者单位： | 厦门出入境检验检疫局; |
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| 摘 要： | 2008年从漳州采集到疑似由柑橘球座菌(Guignardia citricarpaKiely)引起的黑斑病症状的溪琯蜜柚(Citrus grandis(Linn.)Osbeck cv.guanxi-miyou)果实。使用柑橘球座菌(G.citricarpa)特异引物对该果实上的病斑进行PCR检测及序列分析。PCR检测结果表明从溪琯蜜柚果实病斑上扩增到约490bp的条带。序列分析结果表明,与已知的亚洲柑橘叶点霉(Phyllosticta citriasiana)、G.citricarpa和G.mangif-erae的相应序列同源性分别为99.8%、98.2%和94.0%。该序列含有部分的内转录间隔区1(ITS1)、5.8S核糖体RNA基因(5.8S rRNA)和部分的内转录间隔区2(ITS2)序列。结果表明,引起溪琯蜜柚果实病害的病原菌并不是G.citricarpa,而是一种新描述的病原菌——亚洲柑橘叶点霉(Phyllosticta citriasianaWulan-dari,Crous&Gruyter),该病原菌引起的病害称为柑橘褐斑病。
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| 关 键 词： | 琯溪蜜柚 柑橘黑斑病 柑橘褐斑病 亚洲柑橘叶点霉 分子鉴定 |
Molecular identification of the pathogen of black spots on fruit of Citrus grandis (L.) Osbeck cv. guanxi-miyou in Zhangzhou,China |
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| Lin Shiming,Liao Furong,Fang Zhipeng,Huang Pengying,Chen Hongyun,Chen Qing,Wu Yuan.Molecular identification of the pathogen of black spots on fruit of Citrus grandis (L.) Osbeck cv. guanxi-miyou in Zhangzhou,China[J].Plant Quarantine,2010(5). |
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| Authors: | Lin Shiming Liao Furong Fang Zhipeng Huang Pengying Chen Hongyun Chen Qing Wu Yuan |
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| Institution: | Lin Shiming,Liao Furong,Fang Zhipeng,Huang Pengying,Chen Hongyun,Chen Qing,Wu Yuan(Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen 361012,China) |
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| Abstract: | Recently,necrotic spot symptoms similar to those caused by Guignardia citricarpa were observed on fruit of Guanxi Sweet Pomelo(Citrus grandis(L.)Osbeck cv.guanxi-miyou)in Zhangzhou,China.The spot lesions on fruit were detected using species-specific PCR for G.citricarpa and sequence analysis.PCR generated a about 490 bp amplicon from the spot lesions.The nucleotide sequence shared 99.8%,98.2% and 94.0% identities with the internal transcribed spacer(ITS)sequence of Phyllosticta citriasiana,Guignardia citric... |
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| Keywords: | Citrus grandis(L )Osbeck cv guanxi-miyou citrus black spot citrus tan spot Phyllosticta citriasiana molecualr identification |
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