Introgression of in vitro regeneration capability of Lycopersicon pimpinellifolium Mill. into recalcitrant tomato cultivars |
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Authors: | Ricardo Tadeu de Faria Deonisio Destro João Carlos Bespalhok Filho Rolf Dieter Illg |
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Institution: | (1) Department of Agronomy, CCA, State University of Londrina, C.P. 6001, 86051-990 Londrina, PR, Brazil;(2) Biology Institute, State University of Campinas, C.P. 6109, 13083-970 Campinas, SP, Brazil |
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Abstract: | The goal of this research was to study the introgression of the high regeneration capacity of Lycopersicon pimpinellifolium Mill line WV-700, in recalcitrant tomato cultivars (L. esculentum Mill cvs. Petomech, Santa Rita and VFN-8) using backcrossing. Hypocotyl explants of in vitro germinated seeds were cultivated in half strength MS medium supplemented with 5mg/l 6-BA to assess their shoot regeneration
capacity. The apical shoot of the in vitro germinated seeds were cultured on a separate medium. Apical shoots from genotypes showing high regeneration rates were acclimated
in a glasshouse and used as pollen donors for the next backcrossing. After four backcrossings, the material showed a similar
mean fruit weight for the cultivated tomato and a high regeneration capacity similar to the wild species. It is shown that
L. pimpinellifolium can be used with success as donor parent to introgress in vitro regeneration capacity to recalcitrant tomato cultivars.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | interspecific crossing in vitro regeneration Lycopersicon esculentum tissue culture |
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